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Table 1 Yeast strains and plasmids used in this study

From: Increasing n-butanol production with Saccharomyces cerevisiae by optimizing acetyl-CoA synthesis, NADH levels and trans-2-enoyl-CoA reductase expression

Strain or plasmid Characteristics Reference
Strains
 VSY0 MATa; ura3-52; trp1-289; leu2-3_112; his3Δ1; MAL2-8C; SUC2
adh1::loxP adh3::loxP adh5::loxP adh4Δ::loxP adh2Δ::LEU2
[2]
 Y00000 BY4741 MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0 Euroscarf,
Frankfurt
 Y00595 BY4741 Mata; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; fms1::kanMX4 Euroscarf,
Frankfurt
 Y02304 BY4741 MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0;
pan6::kanMX4
Euroscarf,
Frankfurt
 Y06868 BY4741 MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0;
acs1::kanMX4
Euroscarf,
Frankfurt
 JDY2 MATα; his3Δ1; leu2Δ0; lys2Δ0; MET15; ura3Δ0; acs2::kanMX4 Lab stocks,
Boles group
 VSY4 MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; acs1::kanMX4 sfa1Δ:
Ec adhE A267T/E568K/hphNT1
This work
 VSY5 MATα; his3Δ1; leu2Δ0; lys2Δ0; MET15; ura3Δ0; acs2::kanMX4;
sfa1Δ: Ec adhE A267T/E568K, hphNT1
This work
 VSY7 MATα; his3Δ1; leu2Δ0; lys2Δ0; MET15; ura3Δ0; acs2Δ:Ec adhE
A267T/E568K, hphNT1; acs1::kanMX4
This work
 VSY7_evolved MATα; his3Δ1; leu2Δ0; lys2Δ0; MET15; ura3Δ0; acs2Δ:Ec adhE A267T/E568K/R577S, hphNT1; acs1::kanMX4 with unknown mutations for growth on glucose This work
 VSY7_R577S MATα; his3Δ1; leu2Δ0; lys2Δ0; MET15; ura3Δ0; acs2Δ:Ec adhE
A267T/E568K/R577S, hphNT1; acs1::kanMX4
This work
 VSY10 MATa; ura3-52; trp1-289; leu2-3_112; his3Δ1; MAL2-8C; SUC2
adh1::loxP; adh3::loxP: adh5::loxP; adh4Δ::loxP; adh2Δ::LEU2; sfa1Δ: Ec adhE A267T/E568K , hphNT1; adh6Δ: Ec coaA, natNT2; gpd2Δ: Sc ERG10, Ca hbd, Ca crt, Td ter, Ca adhE2, Ec eutE, kanMX
[2]
 VSY13 MATa; ura3-52; trp1-289; leu2-3_112; his3 Δ1; MAL2-8C; SUC2
adh1::loxP; adh3::loxP; adh5::loxP; adh4Δ::loxP; adh2Δ::LEU2; adh6Δ: Ec coaA, loxP; sfa1Δ: Ec adhE A267T/E568K/R577S , loxP; gpd2Δ: Sc ERG10, Ca hbd, Ca crt, Td ter, Ca adhE2, Ec eutE, kanMX
This work
 VSY15 MATa; ura3-52; trp1-289; leu2-3_112; his3 Δ1; MAL2-8C; SUC2
adh1::loxP; adh3::loxP; adh5::loxP; adh4Δ::loxP; adh2Δ::LEU2; adh6Δ: Ec coaA, loxP; sfa1Δ: Ec adhE A267T/E568K/R577S , loxP; pFMS1Δ::HIS3, pADH1; gpd2Δ: Sc ERG10, Ca hbd, Ca crt, Td ter, Ca adhE2, Ec eutE, kanMX
This work
 VSY19 MATa; ura3-52; trp1-289; leu2-3_112; his3 Δ1; MAL2-8C; SUC2
adh1::loxP; adh3::loxP; adh5::loxP; adh4Δ::loxP; adh2Δ::LEU2; adh6Δ:: coaA, loxP; sfa1Δ:: adhE A267T/E568K/R577S , loxP; pFMS1Δ::HIS3, pADH1; ald6Δ; gpd2Δ: Sc ERG10, Ca hbd, Ca crt, Td ter, Ca adhE2, Ec eutE, kanMX
This work
Plasmids
 pRS41H CEN6ARS4, hphNT1, Ampr [29]
 pRS62H , hphNT1, Ampr, shortened HXT7 promoter and CYC1
terminator
[30]
 pVS4 CEN6ARS4, hphNT1, Ampr, Ec adhE A267T/E568K [2]
 pVS4ev CEN6ARS4, hphNT1, Ampr, Ec adhE A267T/E568K/R577S This work
 pRCC-K_ALD6 2µ, kanMX, Ampr, pROX3_Cas9_tCYC1, gRNA for ALD6 This work
 pRS62H_ERG10 , hphNT1, Ampr, Sc ERG10 This work
 pRS62H_hbd , hphNT1, Ampr, Ca hbd This work
 pRS62H_crt , hphNT1, Ampr, Ca crt This work
 pRS62H_ter , hphNT1, Ampr, Td ter This work
 pRS62H_adhE2 , hphNT1, Ampr, Ca adhE2 This work
 pRS62H_eutE , hphNT1, Ampr, Ec eutE This work
 pRS41H_pTPI1_NOX CEN6ARS4, hphNT1, Ampr, NADHoxidase Sp NOX Lab stocks,
Boles group
  1. Genes from Sc, Saccharomyces cerevisiae; Ca, Clostridium acetobutylicum; Ec, E.coli; Td, Treponema denticola; Sp, Streptococcus pneumoniae are indicated by prefixes in superscript. Promoters are indicated in Additional file 1: Table S2. kanMX G418 resistance, hphNT1 hygromycin resistance, Ampr ampicillin resistance