Strain or plasmid | Characteristics | Reference |
---|---|---|
Strains | ||
VSY0 | MATa; ura3-52; trp1-289; leu2-3_112; his3Δ1; MAL2-8C; SUC2 adh1::loxP adh3::loxP adh5::loxP adh4Δ::loxP adh2Δ::LEU2 | [2] |
Y00000 | BY4741 MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0 | Euroscarf, Frankfurt |
Y00595 | BY4741 Mata; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; fms1::kanMX4 | Euroscarf, Frankfurt |
Y02304 | BY4741 MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; pan6::kanMX4 | Euroscarf, Frankfurt |
Y06868 | BY4741 MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; acs1::kanMX4 | Euroscarf, Frankfurt |
JDY2 | MATα; his3Δ1; leu2Δ0; lys2Δ0; MET15; ura3Δ0; acs2::kanMX4 | Lab stocks, Boles group |
VSY4 | MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; acs1::kanMX4 sfa1Δ: Ec adhE A267T/E568K/hphNT1 | This work |
VSY5 | MATα; his3Δ1; leu2Δ0; lys2Δ0; MET15; ura3Δ0; acs2::kanMX4; sfa1Δ: Ec adhE A267T/E568K, hphNT1 | This work |
VSY7 | MATα; his3Δ1; leu2Δ0; lys2Δ0; MET15; ura3Δ0; acs2Δ:Ec adhE A267T/E568K, hphNT1; acs1::kanMX4 | This work |
VSY7_evolved | MATα; his3Δ1; leu2Δ0; lys2Δ0; MET15; ura3Δ0; acs2Δ:Ec adhE A267T/E568K/R577S, hphNT1; acs1::kanMX4 with unknown mutations for growth on glucose | This work |
VSY7_R577S | MATα; his3Δ1; leu2Δ0; lys2Δ0; MET15; ura3Δ0; acs2Δ:Ec adhE A267T/E568K/R577S, hphNT1; acs1::kanMX4 | This work |
VSY10 | MATa; ura3-52; trp1-289; leu2-3_112; his3Δ1; MAL2-8C; SUC2 adh1::loxP; adh3::loxP: adh5::loxP; adh4Δ::loxP; adh2Δ::LEU2; sfa1Δ: Ec adhE A267T/E568K , hphNT1; adh6Δ: Ec coaA, natNT2; gpd2Δ: Sc ERG10, Ca hbd, Ca crt, Td ter, Ca adhE2, Ec eutE, kanMX | [2] |
VSY13 | MATa; ura3-52; trp1-289; leu2-3_112; his3 Δ1; MAL2-8C; SUC2 adh1::loxP; adh3::loxP; adh5::loxP; adh4Δ::loxP; adh2Δ::LEU2; adh6Δ: Ec coaA, loxP; sfa1Δ: Ec adhE A267T/E568K/R577S , loxP; gpd2Δ: Sc ERG10, Ca hbd, Ca crt, Td ter, Ca adhE2, Ec eutE, kanMX | This work |
VSY15 | MATa; ura3-52; trp1-289; leu2-3_112; his3 Δ1; MAL2-8C; SUC2 adh1::loxP; adh3::loxP; adh5::loxP; adh4Δ::loxP; adh2Δ::LEU2; adh6Δ: Ec coaA, loxP; sfa1Δ: Ec adhE A267T/E568K/R577S , loxP; pFMS1Δ::HIS3, pADH1; gpd2Δ: Sc ERG10, Ca hbd, Ca crt, Td ter, Ca adhE2, Ec eutE, kanMX | This work |
VSY19 | MATa; ura3-52; trp1-289; leu2-3_112; his3 Δ1; MAL2-8C; SUC2 adh1::loxP; adh3::loxP; adh5::loxP; adh4Δ::loxP; adh2Δ::LEU2; adh6Δ:: coaA, loxP; sfa1Δ:: adhE A267T/E568K/R577S , loxP; pFMS1Δ::HIS3, pADH1; ald6Δ; gpd2Δ: Sc ERG10, Ca hbd, Ca crt, Td ter, Ca adhE2, Ec eutE, kanMX | This work |
Plasmids | ||
pRS41H | CEN6ARS4, hphNT1, Ampr | [29] |
pRS62H | 2µ, hphNT1, Ampr, shortened HXT7 promoter and CYC1 terminator | [30] |
pVS4 | CEN6ARS4, hphNT1, Ampr, Ec adhE A267T/E568K | [2] |
pVS4ev | CEN6ARS4, hphNT1, Ampr, Ec adhE A267T/E568K/R577S | This work |
pRCC-K_ALD6 | 2µ, kanMX, Ampr, pROX3_Cas9_tCYC1, gRNA for ALD6 | This work |
pRS62H_ERG10 | 2µ, hphNT1, Ampr, Sc ERG10 | This work |
pRS62H_hbd | 2µ, hphNT1, Ampr, Ca hbd | This work |
pRS62H_crt | 2µ, hphNT1, Ampr, Ca crt | This work |
pRS62H_ter | 2µ, hphNT1, Ampr, Td ter | This work |
pRS62H_adhE2 | 2µ, hphNT1, Ampr, Ca adhE2 | This work |
pRS62H_eutE | 2µ, hphNT1, Ampr, Ec eutE | This work |
pRS41H_pTPI1_NOX | CEN6ARS4, hphNT1, Ampr, NADHoxidase Sp NOX | Lab stocks, Boles group |