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Table 1 Yeast strains and plasmids used in this study

From: Increasing n-butanol production with Saccharomyces cerevisiae by optimizing acetyl-CoA synthesis, NADH levels and trans-2-enoyl-CoA reductase expression

Strain or plasmid

Characteristics

Reference

Strains

 VSY0

MATa; ura3-52; trp1-289; leu2-3_112; his3Δ1; MAL2-8C; SUC2

adh1::loxP adh3::loxP adh5::loxP adh4Δ::loxP adh2Δ::LEU2

[2]

 Y00000

BY4741 MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0

Euroscarf,

Frankfurt

 Y00595

BY4741 Mata; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; fms1::kanMX4

Euroscarf,

Frankfurt

 Y02304

BY4741 MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0;

pan6::kanMX4

Euroscarf,

Frankfurt

 Y06868

BY4741 MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0;

acs1::kanMX4

Euroscarf,

Frankfurt

 JDY2

MATα; his3Δ1; leu2Δ0; lys2Δ0; MET15; ura3Δ0; acs2::kanMX4

Lab stocks,

Boles group

 VSY4

MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; acs1::kanMX4 sfa1Δ:

Ec adhE A267T/E568K/hphNT1

This work

 VSY5

MATα; his3Δ1; leu2Δ0; lys2Δ0; MET15; ura3Δ0; acs2::kanMX4;

sfa1Δ: Ec adhE A267T/E568K, hphNT1

This work

 VSY7

MATα; his3Δ1; leu2Δ0; lys2Δ0; MET15; ura3Δ0; acs2Δ:Ec adhE

A267T/E568K, hphNT1; acs1::kanMX4

This work

 VSY7_evolved

MATα; his3Δ1; leu2Δ0; lys2Δ0; MET15; ura3Δ0; acs2Δ:Ec adhE A267T/E568K/R577S, hphNT1; acs1::kanMX4 with unknown mutations for growth on glucose

This work

 VSY7_R577S

MATα; his3Δ1; leu2Δ0; lys2Δ0; MET15; ura3Δ0; acs2Δ:Ec adhE

A267T/E568K/R577S, hphNT1; acs1::kanMX4

This work

 VSY10

MATa; ura3-52; trp1-289; leu2-3_112; his3Δ1; MAL2-8C; SUC2

adh1::loxP; adh3::loxP: adh5::loxP; adh4Δ::loxP; adh2Δ::LEU2; sfa1Δ: Ec adhE A267T/E568K , hphNT1; adh6Δ: Ec coaA, natNT2; gpd2Δ: Sc ERG10, Ca hbd, Ca crt, Td ter, Ca adhE2, Ec eutE, kanMX

[2]

 VSY13

MATa; ura3-52; trp1-289; leu2-3_112; his3 Δ1; MAL2-8C; SUC2

adh1::loxP; adh3::loxP; adh5::loxP; adh4Δ::loxP; adh2Δ::LEU2; adh6Δ: Ec coaA, loxP; sfa1Δ: Ec adhE A267T/E568K/R577S , loxP; gpd2Δ: Sc ERG10, Ca hbd, Ca crt, Td ter, Ca adhE2, Ec eutE, kanMX

This work

 VSY15

MATa; ura3-52; trp1-289; leu2-3_112; his3 Δ1; MAL2-8C; SUC2

adh1::loxP; adh3::loxP; adh5::loxP; adh4Δ::loxP; adh2Δ::LEU2; adh6Δ: Ec coaA, loxP; sfa1Δ: Ec adhE A267T/E568K/R577S , loxP; pFMS1Δ::HIS3, pADH1; gpd2Δ: Sc ERG10, Ca hbd, Ca crt, Td ter, Ca adhE2, Ec eutE, kanMX

This work

 VSY19

MATa; ura3-52; trp1-289; leu2-3_112; his3 Δ1; MAL2-8C; SUC2

adh1::loxP; adh3::loxP; adh5::loxP; adh4Δ::loxP; adh2Δ::LEU2; adh6Δ:: coaA, loxP; sfa1Δ:: adhE A267T/E568K/R577S , loxP; pFMS1Δ::HIS3, pADH1; ald6Δ; gpd2Δ: Sc ERG10, Ca hbd, Ca crt, Td ter, Ca adhE2, Ec eutE, kanMX

This work

Plasmids

 pRS41H

CEN6ARS4, hphNT1, Ampr

[29]

 pRS62H

, hphNT1, Ampr, shortened HXT7 promoter and CYC1

terminator

[30]

 pVS4

CEN6ARS4, hphNT1, Ampr, Ec adhE A267T/E568K

[2]

 pVS4ev

CEN6ARS4, hphNT1, Ampr, Ec adhE A267T/E568K/R577S

This work

 pRCC-K_ALD6

2µ, kanMX, Ampr, pROX3_Cas9_tCYC1, gRNA for ALD6

This work

 pRS62H_ERG10

, hphNT1, Ampr, Sc ERG10

This work

 pRS62H_hbd

, hphNT1, Ampr, Ca hbd

This work

 pRS62H_crt

, hphNT1, Ampr, Ca crt

This work

 pRS62H_ter

, hphNT1, Ampr, Td ter

This work

 pRS62H_adhE2

, hphNT1, Ampr, Ca adhE2

This work

 pRS62H_eutE

, hphNT1, Ampr, Ec eutE

This work

 pRS41H_pTPI1_NOX

CEN6ARS4, hphNT1, Ampr, NADHoxidase Sp NOX

Lab stocks,

Boles group

  1. Genes from Sc, Saccharomyces cerevisiae; Ca, Clostridium acetobutylicum; Ec, E.coli; Td, Treponema denticola; Sp, Streptococcus pneumoniae are indicated by prefixes in superscript. Promoters are indicated in Additional file 1: Table S2. kanMX G418 resistance, hphNT1 hygromycin resistance, Ampr ampicillin resistance