Fig. 3

Growth curves of gene deletion mutants. a Mutants lacking enzymes, b mutants lacking the two-component regulation system and inner membrane sugar transporter, c mutants lacking individual CusC and CusD proteins, and mutants lacking both CusC or both CusD proteins, and d mutants lacking CusC/CusD pairs. pSSK05 expresses ChiA, pYT259 expresses the two-component signal transduction proteins CusS and CusR, pYT260 expresses the predicted cytoplasmic membrane sugar transporter Fjoh_4565, pLW01 expresses CusD_I, pYT351 expresses CusC_I and CusD_I, and pYT352 expresses CusC_II and CusD_II. Cells (0.1 ml, OD₆₀₀ = 1.0) were introduced into 50 ml of Stanier medium supplemented with 0.05% chitin in 250-ml flasks and incubated with shaking at 25 °C. Growth presented as log (µg cell protein/ml). Growth experiments were performed in triplicate and error bars indicate standard deviations