Fig. 5

Chitin degradation by the ChiUL. The ChiUL-encoded chitinase enzymes were incubated with β-chitin (a) or α-chitin (b) at 5 g/l, respectively. Enzyme reactions are labeled as follows: black circles—ChiA_F (1 µM), red squares—ChiA_N (10 µM), green triangles—ChiA_C (10 µM), purples crosses—ChiB (10 µM), and blue diamonds—synergy reaction (ChiA_N & ChiA_C; 5 µM each). Additional enzyme was added after 27 h, indicated by large arrows (24 h for ChiB, small arrows). No observable hydrolytic activity could be detected by ChiA_M. Data points are the average of triplicate experiments and error bars indicate standard errors