Fig. 6From: A polysaccharide utilization locus from Flavobacterium johnsoniae enables conversion of recalcitrant chitinBinding analysis of ChiA_M to insoluble polysaccharides. Lanes control, protein without substrate, U unbound protein, W wash fraction, E proteins eluted by incubation with urea. Lanes marked with an asterisk in the upper row refer to experiments using ball-milled chitin, while unmarked lanes refer to crushed chitin, sieved to a particle size of ≤0.85 mmBack to article page