Fig. 6

Binding analysis of ChiA_M to insoluble polysaccharides. Lanes control, protein without substrate, U unbound protein, W wash fraction, E proteins eluted by incubation with urea. Lanes marked with an asterisk in the upper row refer to experiments using ball-milled chitin, while unmarked lanes refer to crushed chitin, sieved to a particle size of ≤0.85 mm