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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Systematic engineering of pentose phosphate pathway improves Escherichia coli succinate production

Fig. 2

Schematic of the construction of ribosome binding site library (RBSL) of PPP enzymes. The seven PPP enzymes locate dispersedly among E. coli MG1655 chromosome. A constitutive promoter M1-93 was initially used to replace native promoter of PPP enzymes. Next, seven degenerate nucleotides (RNNNNNN) were introduced into the RBS region of M1-93 promoter before ATG start codon to obtain the RBSL

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