Fig. 2From: Systematic engineering of pentose phosphate pathway improves Escherichia coli succinate productionSchematic of the construction of ribosome binding site library (RBSL) of PPP enzymes. The seven PPP enzymes locate dispersedly among E. coli MG1655 chromosome. A constitutive promoter M1-93 was initially used to replace native promoter of PPP enzymes. Next, seven degenerate nucleotides (RNNNNNN) were introduced into the RBS region of M1-93 promoter before ATG start codon to obtain the RBSLBack to article page