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Table 1 Strains and plasmids used in this study

From: Enhanced production of 2,3-butanediol by engineered Saccharomyces cerevisiae through fine-tuning of pyruvate decarboxylase and NADH oxidase activities

Strains and plasmids Description Reference
Strains
 Candida tropicalis ATCC20336 Source for CtPDC1 In this study
 Kluyveromyces marxianus KCTC17555 Source for KmPDC1 In this study
 Saccharomyces cerevisiae D452-2 Source for ScPDC1, ScPDC5, ScPDC6 MATα leu2 his3 ura3 [49]
 BD4 D452-2, pdc1Δ, pdc5Δ, evolved 2,3-BD-producing strain [12]
 SOS5 D452-2, pdc1Δ, pdc5Δ, pdc6Δ [18]
 SOS5_T2CtPDC1 SOS5, p426TDH3_CtPDC1 In this study
 SOS5_T2KmPDC1 SOS5, p426TDH3_KmPDC1 In this study
 SOS5_T2ScPDC1 SOS5, p426TDH3_ScPDC1 In this study
 SOS5_T2ScPDC5 SOS5, p426TDH3_ScPDC5 In this study
 SOS5_T2ScPDC6 SOS5, p426TDH3_ScPDC6 In this study
 BD5 SOS5, p423_alsSalsD, p425_BDH1 [18]
 BD5_Con BD5, p426GPD [18]
 BD5_C1CtPDC1 BD5, p406CYC1_CtPDC1 In this study
 BD5_G1CtPDC1 BD5, p406GPD2_CtPDC1 In this study
 BD5_C2CtPDC1 BD5, p426CYC1_CtPDC1 In this study
 BD5_T2CtPDC1 BD5, p426TDH3_CtPDC1 In this study
 BD5_G1CtPDC1_nox BD5, p406GPD2_CtPDC1, pAUR_Llnox In this study
Plasmids
 pRS406 URA3 [50]
 pRS426 URA3 2 μm origin [50]
 p406GPD2 pRS406, GPD2 prom CYC1 term [18]
 p426CYC1 pRS426, CYC1 prom CYC1 term [18]
 p426GPD URA3 2 μm origin, TDH3 prom CYC1 term [50]
 p423_alsSalsD HIS3 2 μm origin, TDH3 prom-alsS-CYC1 term TDH3 prom-alsD-CYC1 term [51]
 p425_BDH1 LEU2 2 μm origin, TDH3 prom-BDH1-CYC1 term [12]
 p426TDH3_Llnox pRS426, TDH3 prom-Llnox-CYC1 term [18]
 p426TDH3_CtPDC1 pRS426, TDH3 prom-CtPDC1-CYC1 term In this study
 p426TDH3_KmPDC1 pRS426, TDH3 prom-KmPDC1-CYC1 term In this study
 p426TDH3_ScPDC1 pRS426, TDH3 prom-ScPDC1-CYC1 term In this study
 p426TDH3_ScPDC5 pRS426, TDH3 prom-ScPDC5-CYC1 term In this study
 p426TDH3_ScPDC6 pRS426, TDH3 prom-ScPDC6-CYC1 term In this study
 p406CYC1_CtPDC1 pRS406, CYC1 prom-CtPDC1-CYC1 term In this study
 p406GPD2_CtPDC1 pRS406, GPD2 prom-CtPDC1-CYC1 term In this study
 p426CYC1_CtPDC1 pRS426, CYC1 prom-CtPDC1-CYC1 term In this study
 pAUR_Llnox AUR1-C CEN6 ARS4, TDH3 prom-Llnox-CYC1 term In this study