Skip to main content

Table 2 Primers used in this study

From: Enhanced production of 2,3-butanediol by engineered Saccharomyces cerevisiae through fine-tuning of pyruvate decarboxylase and NADH oxidase activities

Primers

Restriction site

Sequence

Cloning of PDC genes

 F_XmaI_CtPDC1

Xma I

tcccCCCGGGaaaatgtctgaaattactttgggtag

 R_SalI_CtPDC1

Sal I

acgcGTCGACtttattcttgagcagcgttg

 F_XmaI_KmPDC1

Xma I

tcccCCCGGGaaaatgtctgaaattactctaggtcg

 R_SalI_KmPDC1

Sal I

acgcGTCGACtttattcttgcttggcgtt

 F_XmaI_ScPDC1

Xma I

tcccCCCGGGatgtctgaaattactttgggtaa

 R_SalI_ScPDC1

Sal I

acgcGTCGACttattgcttagcgttggtag

 F_XmaI_ScPDC5

Xma I

tcccCCCGGGatgtctgaaataaccttaggtaaata

 R_SalI_ScPDC5

Sal I

acgcGTCGACttattgtttagcgttagtagcg

 F_XmaI_ScPDC6

Xma I

tcccCCCGGGatgtctgaaattactcttggaaaatac

 R_SalI_ScPDC6

Sal I

acgcGTCGACttattgtttggcatttgtagc

 Cloning of AUR1-C gene

 F_SnaBI_AUR1-C

SnaB I

agcttgtcacctTACGTAaaagtgcccatcagtgttc

 R_MfeI_AUR1-C

Mfe I

ataaccgggtCAATTGcagaggaaagaataacgcaa

  1. Bold and capital characters are restriction enzyme sites