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Table 3 Thermotoga grown in the presence of different concentrations of thiosulfate

From: Hydrogen production by the hyperthermophilic bacterium Thermotoga maritima part I: effects of sulfured nutriments, with thiosulfate as model, on hydrogen production and growth

Thiosulfate Time Cells Glu cons Lactate Acetate CO2 H2 C1-recovery l-Alanine EPS C2-recovery
m mol h mg L−1 m mol % mmol %
0.0 t 0 0.0 19.3 ± 2.2 0.0 0.0 0.0 0.0 0.0 0.0 0.0
t 1 4.8 31.8 ± 2.5 1.3 ± 0.3 0.2 ± 0.0 0.5 ± 0.2 0.2 ± 0.1 0.3 ± 0.0
t 2 29.5 127.6 ± 9.7 16.4 ± 1.1 4.9 ± 0.5 11.8 ± 0.8 11.1 ± 1.0 23.2 ± 2.0 54.9 ± 12.1 1.39 ± 0.2 4.7 ± 1.2 87.2 ± 18.1
t 3 30.6 119.8 ± 10.9 17.7 ± 1.9 5.4 ± 0.6 12.8 ± 1.0 11.9 ± 1.1 25.0 ± 2.2 54.5 ± 12.0
0.01 t 0 0.0 16.8 ± 3.1 0.0 0.0 0.0 0.0 0.0
t 1 3.1 22.8 ± 1.7 2.1 ± 0.5 0.1 ± 0.0 0.2 ± 0.0 0.2 ± 0.0 0.4 ± 0.0
t 2 22.8 178.0 ± 15.4 19.8 ± 1.1 10.0 ± 1.0 16.0 ± 0.8 15.0 ± 1.2 30.7 ± 2.3 70.4 ± 11.7
t 3 23.0 177.4 ± 15.2 20.0 ± 1.1 10.2 ± 1.1 16.0 ± 0.8 15.0 ± 1.2 31.0 ± 2.3 70.6 ± 11.7
0.03 t 0 0.0 23.6 ± 3.9 0.0 0.0 0.0 0.0 0.0
t 1 6.7 32.1 ± 3.0 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0 0.2 ± 0.0 0.3 ± 0.0
t 2 24.1 265.0 ± 22.5 25.2 ± 2.3 6.8 ± 1.9 27.5 ± 2.1 27.3 ± 2.6 53.5 ± 4.6 74.6 ± 12.4
t 3 27.5 264.1 ± 22.4 28.0 ± 1.5 8.2 ± 0.7 30.6 ± 1.9 29.7 ± 2.5 57.9 ± 4.8 74.7 ± 12.4
0.06 t0 0.0 26.1 ± 2.8 0.0 0.0 0.0 0.0 0.0
t 1 1.8 33.7 ± 4.0 0.7 ± 0.2 0.0 ± 0.0 0.1 ± 0.0 0.1 ± 0.1 0.3 ± 0.0
t 2 20.4 353.5 ± 26.5 38.2 ± 2.0 18.0 ± 1.8 37.7 ± 2.3 35.7 ± 3.0 73.2 ± 5.9 78.1 ± 13.0
t 3 22.3 352.3 ± 26.4 38.5 ± 2.0 18.1 ± 1.8 38.2 ± 2.4 35.8 ± 3.0 73.3 ± 5.9 77.9 ± 12.9
0.12 t 0 0.0 24.4 ± 2.3 0.0 0.0 0.0 0.0 0.0 0.0 0.0
t 1 3.0 34.1 ± 2.8 1.1 ± 0.3 0.0 ± 0.0 0.8 ± 0.4 0.3 ± 0.1 0.3 ± 0.0
t 2 17.9 404.0 ± 32.3 42.4 ± 2.3 15.9 ± 1.5 47.0 ± 2.5 44.2 ± 3.7 90.5 ± 7.0 79.2 ± 13.2 3.8 ± 0.3 3.6 ± 0.9 92.2 ± 19.0
t 3 23.2 396.6 ± 32.8 45.7 ± 2.5 15.4 ± 1.6 52.4 ± 3.3 51.9 ± 4.9 99.7 ± 8.3 79.6 ± 13.2
0.18 t 0 0.0 25.3 ± 2.5 0.0 0.0 0.0 0.0 0.0
t 1 1.2 32.7 ± 3.3 0.3 ± 0 0.0 ± 0 0.0 ± 0 0.4 ± 0.1 0.4 ± 0.1
t 2 16.8 428.8 ± 32.2 45.0 ± 2.2 23.3 ± 2.3 44.5 ± 2.5 44.6 ± 4.0 86.7 ± 7.0 81.5 ± 13.5
t 3 17.0 418.0 ± 33.0 45.4 ± 2.3 23.4 ± 2.3 45.0 ± 2.2 44.7 ± 5.0 86.9 ± 8.2 81.3 ± 13.5
0.24 t 0 0.0 27.5 ± 2.1 0.0 0.0 0.0 0.0 0.0 0.0 0.0
t 1 2.1 33.8 ± 2.6 0.9 ± 0.3 0.0 ± 0.0 0.0 ± 0.0 0.2 ± 0.0 0.5 ± 0.0
t 2 17.0 423.9 ± 31.8 41.7 ± 2.1 22.5 ± 1.5 43.4 ± 3.5 40.0 ± 3.2 84.0 ± 7.0 84.3 ± 14.0 3.8 ± 0.2 2.9 ± 0.7 95.8 ± 19.8
t 3 22.8 422.4 ± 31.7 43.8 ± 2.2 26.4 ± 1.4 46.1 ± 3.3 42.2 ± 3.8 88.6 ± 8.9 87.5 ± 14.5
  1. q glucose and q H2 were the specific glucose consumption rate and the specific hydrogen productivity, respectively. They were calculated in taking into account the linear increase of cell concentration found during the growth phases (from t1 to t2). They were expressed in mmol per g of cells (dw) and per hour
Thiosulfate Time Cells/glu Acet/glu Lac/glu H2/glu l-ala/glu EPS/glu q glucose q H2
m mol h g mol−1   mol mol−1 mmol g−1 h−1
0.0 t 0 0.0
t 1 4.8
t 2 29.5 6.3 ± 0.6 0.7 ± 0.1 0.3 ± 0.0 1.4 ± 0.2 0.08 ± 0.01 0.29 ± 0.05 8.9 ± 1.3 13.4 ± 2.3
t 3 30.6 0.7 ± 0.1 0.3 ± 0.0 1.4 ± 0.2
0.01 t 0 0.0
t 1 3.1
t 2 22.8 8.7 ± 0.9 0.8 ± 0.1 0.5 ± 0.0 1.6 ± 0.2 12.0 ± 1.8 20.4 ± 3.5
t 3 23.0 0.8 ± 0.1 0.5 ± 0.1 1.6 ± 0.2
0.03 t 0 0.0
t 1 6.7
t 2 24.1 9.3 ± 1.4 1.1 ± 0.1 0.3 ± 0.1 2.1 ± 0.2 13.1 ± 2.0 27.7 ± 4.7
t 3 27.5 1.1 ± 0.1 0.3 ± 0.1 2.1 ± 0.2
0.06 t 0 0.0
t 1 1.8
t 2 20.4 8.5 ± 0.8 1.0 ± 0.1 0.5 ± 0.0 1.9 ± 0.2 14.8 ± 2.2 28.7 ± 4.9
t 3 22.3 1.0 ± 0.1 0.5 ± 0.0 1.9 ± 0.2
0.12 t 0 0.0
t 1 3.0
t 2 17.9 8.9 ± 0.8 1.1 ± 0.1 0.4 ± 0.0 2.1 ± 0.2 0.09 ± 0.01 0.08 ± 0.01 18.5 ± 2.8 40.4 ± 6.9
t 3 23.2 1.1 ± 0.1 0.3 ± 0.0 2.2 ± 0.2
0.18 t 0 0.0
t 1 1.2
t 2 16.8 8.7 ± 0 1.0 ± 0.1 0.5 ± 0.0 1.9 ± 0.2 18.8 ± 2.8 36.3 ± 6.2
t 3 17.0 1.0 ± 0.1 0.5 ± 0.0 1.9 ± 0.2
0.24 t 0 0.0
t 1 2.1
t 2 17.0 9.6 ± 0.9 1.0 ± 0.1 0.5 ± 0.0 2.0 ± 0.2 0.09 ± 0.01 0.07 ± 0.01 17.9 ± 2.7 36.6 ± 6.2
t 3 22.8 1.1 ± 0.1 0.6 ± 0.0 2.0 ± 0.2
  1. q glucose and q H2 were the specific glucose consumption rate and the specific hydrogen productivity, respectively. They were calculated in taking into account the linear increase of cell concentration found during the growth phases (from t1 to t2). They were expressed in mmol per g of cells (dw) and per hour