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Table 3 Thermotoga grown in the presence of different concentrations of thiosulfate

From: Hydrogen production by the hyperthermophilic bacterium Thermotoga maritima part I: effects of sulfured nutriments, with thiosulfate as model, on hydrogen production and growth

Thiosulfate

Time

Cells

Glu cons

Lactate

Acetate

CO2

H2

C1-recovery

l-Alanine

EPS

C2-recovery

m mol

h

mg L−1

m mol

%

mmol

%

0.0

t 0

0.0

19.3 ± 2.2

0.0

0.0

0.0

0.0

0.0

–

0.0

0.0

–

t 1

4.8

31.8 ± 2.5

1.3 ± 0.3

0.2 ± 0.0

0.5 ± 0.2

0.2 ± 0.1

0.3 ± 0.0

–

–

–

–

t 2

29.5

127.6 ± 9.7

16.4 ± 1.1

4.9 ± 0.5

11.8 ± 0.8

11.1 ± 1.0

23.2 ± 2.0

54.9 ± 12.1

1.39 ± 0.2

4.7 ± 1.2

87.2 ± 18.1

t 3

30.6

119.8 ± 10.9

17.7 ± 1.9

5.4 ± 0.6

12.8 ± 1.0

11.9 ± 1.1

25.0 ± 2.2

54.5 ± 12.0

–

–

–

0.01

t 0

0.0

16.8 ± 3.1

0.0

0.0

0.0

0.0

0.0

–

–

–

–

t 1

3.1

22.8 ± 1.7

2.1 ± 0.5

0.1 ± 0.0

0.2 ± 0.0

0.2 ± 0.0

0.4 ± 0.0

–

–

–

–

t 2

22.8

178.0 ± 15.4

19.8 ± 1.1

10.0 ± 1.0

16.0 ± 0.8

15.0 ± 1.2

30.7 ± 2.3

70.4 ± 11.7

–

–

–

t 3

23.0

177.4 ± 15.2

20.0 ± 1.1

10.2 ± 1.1

16.0 ± 0.8

15.0 ± 1.2

31.0 ± 2.3

70.6 ± 11.7

–

–

–

0.03

t 0

0.0

23.6 ± 3.9

0.0

0.0

0.0

0.0

0.0

–

–

–

–

t 1

6.7

32.1 ± 3.0

0.0 ± 0.0

0.0 ± 0.0

0.0 ± 0.0

0.2 ± 0.0

0.3 ± 0.0

–

–

–

–

t 2

24.1

265.0 ± 22.5

25.2 ± 2.3

6.8 ± 1.9

27.5 ± 2.1

27.3 ± 2.6

53.5 ± 4.6

74.6 ± 12.4

–

–

–

t 3

27.5

264.1 ± 22.4

28.0 ± 1.5

8.2 ± 0.7

30.6 ± 1.9

29.7 ± 2.5

57.9 ± 4.8

74.7 ± 12.4

–

–

–

0.06

t0

0.0

26.1 ± 2.8

0.0

0.0

0.0

0.0

0.0

–

–

–

–

t 1

1.8

33.7 ± 4.0

0.7 ± 0.2

0.0 ± 0.0

0.1 ± 0.0

0.1 ± 0.1

0.3 ± 0.0

–

–

–

–

t 2

20.4

353.5 ± 26.5

38.2 ± 2.0

18.0 ± 1.8

37.7 ± 2.3

35.7 ± 3.0

73.2 ± 5.9

78.1 ± 13.0

–

–

–

t 3

22.3

352.3 ± 26.4

38.5 ± 2.0

18.1 ± 1.8

38.2 ± 2.4

35.8 ± 3.0

73.3 ± 5.9

77.9 ± 12.9

–

–

–

0.12

t 0

0.0

24.4 ± 2.3

0.0

0.0

0.0

0.0

0.0

–

0.0

0.0

–

t 1

3.0

34.1 ± 2.8

1.1 ± 0.3

0.0 ± 0.0

0.8 ± 0.4

0.3 ± 0.1

0.3 ± 0.0

–

–

–

–

t 2

17.9

404.0 ± 32.3

42.4 ± 2.3

15.9 ± 1.5

47.0 ± 2.5

44.2 ± 3.7

90.5 ± 7.0

79.2 ± 13.2

3.8 ± 0.3

3.6 ± 0.9

92.2 ± 19.0

t 3

23.2

396.6 ± 32.8

45.7 ± 2.5

15.4 ± 1.6

52.4 ± 3.3

51.9 ± 4.9

99.7 ± 8.3

79.6 ± 13.2

–

–

–

0.18

t 0

0.0

25.3 ± 2.5

0.0

0.0

0.0

0.0

0.0

–

–

–

–

t 1

1.2

32.7 ± 3.3

0.3 ± 0

0.0 ± 0

0.0 ± 0

0.4 ± 0.1

0.4 ± 0.1

–

–

–

–

t 2

16.8

428.8 ± 32.2

45.0 ± 2.2

23.3 ± 2.3

44.5 ± 2.5

44.6 ± 4.0

86.7 ± 7.0

81.5 ± 13.5

–

–

–

t 3

17.0

418.0 ± 33.0

45.4 ± 2.3

23.4 ± 2.3

45.0 ± 2.2

44.7 ± 5.0

86.9 ± 8.2

81.3 ± 13.5

–

–

–

0.24

t 0

0.0

27.5 ± 2.1

0.0

0.0

0.0

0.0

0.0

–

0.0

0.0

–

t 1

2.1

33.8 ± 2.6

0.9 ± 0.3

0.0 ± 0.0

0.0 ± 0.0

0.2 ± 0.0

0.5 ± 0.0

–

–

–

–

t 2

17.0

423.9 ± 31.8

41.7 ± 2.1

22.5 ± 1.5

43.4 ± 3.5

40.0 ± 3.2

84.0 ± 7.0

84.3 ± 14.0

3.8 ± 0.2

2.9 ± 0.7

95.8 ± 19.8

t 3

22.8

422.4 ± 31.7

43.8 ± 2.2

26.4 ± 1.4

46.1 ± 3.3

42.2 ± 3.8

88.6 ± 8.9

87.5 ± 14.5

–

–

–

Thiosulfate

Time

Cells/glu

Acet/glu

Lac/glu

H2/glu

l-ala/glu

EPS/glu

q glucose

q H2

m mol

h

g mol−1

 

mol mol−1

mmol g−1 h−1

0.0

t 0

0.0

–

–

–

–

–

–

–

–

t 1

4.8

–

–

–

–

–

–

–

–

t 2

29.5

6.3 ± 0.6

0.7 ± 0.1

0.3 ± 0.0

1.4 ± 0.2

0.08 ± 0.01

0.29 ± 0.05

8.9 ± 1.3

13.4 ± 2.3

t 3

30.6

–

0.7 ± 0.1

0.3 ± 0.0

1.4 ± 0.2

–

–

–

–

0.01

t 0

0.0

–

–

–

–

–

–

–

–

t 1

3.1

–

–

–

–

–

–

–

–

t 2

22.8

8.7 ± 0.9

0.8 ± 0.1

0.5 ± 0.0

1.6 ± 0.2

–

–

12.0 ± 1.8

20.4 ± 3.5

t 3

23.0

–

0.8 ± 0.1

0.5 ± 0.1

1.6 ± 0.2

–

–

–

–

0.03

t 0

0.0

–

–

–

–

–

–

–

–

t 1

6.7

–

–

–

–

–

–

–

–

t 2

24.1

9.3 ± 1.4

1.1 ± 0.1

0.3 ± 0.1

2.1 ± 0.2

–

–

13.1 ± 2.0

27.7 ± 4.7

t 3

27.5

–

1.1 ± 0.1

0.3 ± 0.1

2.1 ± 0.2

–

–

–

–

0.06

t 0

0.0

–

–

–

–

–

–

–

–

t 1

1.8

–

–

–

–

–

–

–

–

t 2

20.4

8.5 ± 0.8

1.0 ± 0.1

0.5 ± 0.0

1.9 ± 0.2

–

–

14.8 ± 2.2

28.7 ± 4.9

t 3

22.3

–

1.0 ± 0.1

0.5 ± 0.0

1.9 ± 0.2

–

–

–

–

0.12

t 0

0.0

–

–

–

–

–

–

–

–

t 1

3.0

–

–

–

–

–

–

–

–

t 2

17.9

8.9 ± 0.8

1.1 ± 0.1

0.4 ± 0.0

2.1 ± 0.2

0.09 ± 0.01

0.08 ± 0.01

18.5 ± 2.8

40.4 ± 6.9

t 3

23.2

–

1.1 ± 0.1

0.3 ± 0.0

2.2 ± 0.2

–

–

–

–

0.18

t 0

0.0

–

–

–

–

–

–

–

–

t 1

1.2

–

–

–

–

–

–

–

–

t 2

16.8

8.7 ± 0

1.0 ± 0.1

0.5 ± 0.0

1.9 ± 0.2

–

–

18.8 ± 2.8

36.3 ± 6.2

t 3

17.0

–

1.0 ± 0.1

0.5 ± 0.0

1.9 ± 0.2

–

–

–

–

0.24

t 0

0.0

–

–

–

–

–

–

–

–

t 1

2.1

–

–

–

–

–

–

–

–

t 2

17.0

9.6 ± 0.9

1.0 ± 0.1

0.5 ± 0.0

2.0 ± 0.2

0.09 ± 0.01

0.07 ± 0.01

17.9 ± 2.7

36.6 ± 6.2

t 3

22.8

–

1.1 ± 0.1

0.6 ± 0.0

2.0 ± 0.2

–

–

–

–

  1. q glucose and q H2 were the specific glucose consumption rate and the specific hydrogen productivity, respectively. They were calculated in taking into account the linear increase of cell concentration found during the growth phases (from t1 to t2). They were expressed in mmol per g of cells (dw) and per hour