Succinate production positively correlates with the affinity of the global transcription factor Cra for its effector FBP in Escherichia coli

Wei, Li-Na; Zhu, Li-Wen; Tang, Ya-Jie

doi:10.1186/s13068-016-0679-7

Biotechnology for Biofuels and Bioproducts

Table 1 The characteristic of single-point mutations, mutation energy, and succinate production

From: Succinate production positively correlates with the affinity of the global transcription factor Cra for its effector FBP in Escherichia coli

Strain	Residue	Mutated to	Mutation energy (kcal/mol)	Effect	Succinate production (g/L)
Tang1646	Asp148	Arg	−3.15	Stabilizing	33.8
Tang1647	Gly274	Arg	−3.04	Stabilizing	33.2
Tang1648	Asp101	Arg	−2.84	Stabilizing	36.7
Tang1649	Ser75	Lys	−2.60	Stabilizing	35.0
Tang1650	Tyr220	Lys	−2.40	Stabilizing	32.7
Tang1651	Asp148	Lys	−2.34	Stabilizing	34.8
Tang1652	Leu191	Lys	−2.04	Stabilizing	34.2
Tang1653	Asn73	Tyr	−1.92	Stabilizing	32.2
Tang1654	Ser246	Lys	−1.90	Stabilizing	32.1
Tang1655	Phe273	Lys	−1.58	Stabilizing	31.6
Tang1656	Tyr76	Arg	−1.51	Stabilizing	31.6
Tang1657	Asp275	Glu	−1.50	Stabilizing	32.5
Tang1658	Gln291	Arg	−1.32	Stabilizing	32.2
Tang1659	Ala248	Lys	−1.22	Stabilizing	33.9
Tang1660	Arg323	Lys	−1.06	Stabilizing	31.7
Tang1661	Asn73	Arg	−1.05	Stabilizing	32.6
Tang1662	Ser75	Arg	−1.04	Stabilizing	31.3
Tang1663	Thr245	Ser	−1.04	Stabilizing	30.6
Tang1664	Asp275	Gln	−1.02	Stabilizing	30.5
Tang1665	Asn73	Phe	−0.84	Stabilizing	31.8
Tang1666	Lys322	Asp	−0.84	Stabilizing	32.7
Tang1667	Phe247	Pro	−0.81	Stabilizing	32.4
Tang1668	Thr74	Lys	−0.75	Stabilizing	32.0
Tang1669	Ala248	Phe	−0.72	Stabilizing	30.5
Tang1670	Arg149	Lys	−0.67	Stabilizing	31.4
Tang1671	Asp148	Glu	−0.40	Destabilizing	33.1
Tang1672	Tyr220	Pro	−0.22	Destabilizing	32.0
Tang1673	Tyr220	Leu	−0.20	Destabilizing	29.0
Tang1674	Arg197	Lys	−0.04	Destabilizing	30.4
Tang1675	Phe247	Glu	0.38	Destabilizing	31.3
Tang1676	Tyr76	Asp	0.95	Destabilizing	30.1
Tang1677	Phe247	Arg	1.08	Destabilizing	29.3
Tang1678	Ser75	Glu	1.65	Destabilizing	27.0
Tang1679	Arg149	Phe	1.65	Destabilizing	29.8
Tang1680	Arg149	His	2.24	Destabilizing	27.2
Tang1681	Gln291	Met	2.54	Destabilizing	25.7
Tang1682	Gln291	Trp	3.57	Destabilizing	24.3

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