Skip to main content

Advertisement

Table 1 The characteristic of single-point mutations, mutation energy, and succinate production

From: Succinate production positively correlates with the affinity of the global transcription factor Cra for its effector FBP in Escherichia coli

Strain Residue Mutated to Mutation energy (kcal/mol) Effect Succinate production (g/L)
Tang1646 Asp148 Arg −3.15 Stabilizing 33.8
Tang1647 Gly274 Arg −3.04 Stabilizing 33.2
Tang1648 Asp101 Arg −2.84 Stabilizing 36.7
Tang1649 Ser75 Lys −2.60 Stabilizing 35.0
Tang1650 Tyr220 Lys −2.40 Stabilizing 32.7
Tang1651 Asp148 Lys −2.34 Stabilizing 34.8
Tang1652 Leu191 Lys −2.04 Stabilizing 34.2
Tang1653 Asn73 Tyr −1.92 Stabilizing 32.2
Tang1654 Ser246 Lys −1.90 Stabilizing 32.1
Tang1655 Phe273 Lys −1.58 Stabilizing 31.6
Tang1656 Tyr76 Arg −1.51 Stabilizing 31.6
Tang1657 Asp275 Glu −1.50 Stabilizing 32.5
Tang1658 Gln291 Arg −1.32 Stabilizing 32.2
Tang1659 Ala248 Lys −1.22 Stabilizing 33.9
Tang1660 Arg323 Lys −1.06 Stabilizing 31.7
Tang1661 Asn73 Arg −1.05 Stabilizing 32.6
Tang1662 Ser75 Arg −1.04 Stabilizing 31.3
Tang1663 Thr245 Ser −1.04 Stabilizing 30.6
Tang1664 Asp275 Gln −1.02 Stabilizing 30.5
Tang1665 Asn73 Phe −0.84 Stabilizing 31.8
Tang1666 Lys322 Asp −0.84 Stabilizing 32.7
Tang1667 Phe247 Pro −0.81 Stabilizing 32.4
Tang1668 Thr74 Lys −0.75 Stabilizing 32.0
Tang1669 Ala248 Phe −0.72 Stabilizing 30.5
Tang1670 Arg149 Lys −0.67 Stabilizing 31.4
Tang1671 Asp148 Glu −0.40 Destabilizing 33.1
Tang1672 Tyr220 Pro −0.22 Destabilizing 32.0
Tang1673 Tyr220 Leu −0.20 Destabilizing 29.0
Tang1674 Arg197 Lys −0.04 Destabilizing 30.4
Tang1675 Phe247 Glu 0.38 Destabilizing 31.3
Tang1676 Tyr76 Asp 0.95 Destabilizing 30.1
Tang1677 Phe247 Arg 1.08 Destabilizing 29.3
Tang1678 Ser75 Glu 1.65 Destabilizing 27.0
Tang1679 Arg149 Phe 1.65 Destabilizing 29.8
Tang1680 Arg149 His 2.24 Destabilizing 27.2
Tang1681 Gln291 Met 2.54 Destabilizing 25.7
Tang1682 Gln291 Trp 3.57 Destabilizing 24.3