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Table 2 The characteristic of three-point mutations, mutation energy, and succinate production

From: Succinate production positively correlates with the affinity of the global transcription factor Cra for its effector FBP in Escherichia coli

Strain Mutated to Mutation energy (kcal/mol) Succinate production (g/L)
Tang1683 Asp101Arg + Asp148Arg + Gly274Arg −4.78 92.7
Tang1684 Asp148Arg + Ala248Phe + Gly274Arg −4.29 90.0
Tang1685 Asp148Arg + Tyr220Lys + Gly274Arg −4.19 79.1
Tang1686 Asp101Arg + Asp148Arg + Ser246Lys −4.03 79.3
Tang1687 Asp148Arg + Gly274Arg + Asp275Gln −3.99 79.4
Tang1688 Asn73Tyr + Asp148Arg + Gly274Arg −3.89 88.3
Tang1689 Ser75Lys + Ala248Phe + Gly274Arg −3.75 77.5
Tang1690 Asp148Arg + Thr245Ser + Gly274Arg −3.68 78.9
Tang1691 Asp148Arg + Ser246Lys + Gly274Arg −3.63 73.7
Tang1692 Ser75Lys + Ser246Lys + Gly274Arg −3.45 75.0
Tang1693 Asp148Arg + Ser246Lys + Phe247Pro −3.36 73.5
Tang1694 Asp148Arg + Phe273Lys + Gly274Arg −3.36 73.6
Tang1695 Asp148Arg + Tyr220Lys + Ser246Lys −3.33 69.8
Tang1696 Asp148Arg + Phe247Pro + Gly274Arg −3.22 70.3
Tang1697 Asp148Arg + Ser246Lys + Ala248Phe −3.18 70.3
Tang1698 Ser75Lys + Phe273Lys + Gly274Arg −3.17 70.1
Tang1699 Ser75Lys + Gly274Arg + Asp275Gln −3.16 69.8