Table 2 The characteristic of three-point mutations, mutation energy, and succinate production
Strain | Mutated to | Mutation energy (kcal/mol) | Succinate production (g/L) |
|---|---|---|---|
Tang1683 | Asp101Arg + Asp148Arg + Gly274Arg | −4.78 | 92.7 |
Tang1684 | Asp148Arg + Ala248Phe + Gly274Arg | −4.29 | 90.0 |
Tang1685 | Asp148Arg + Tyr220Lys + Gly274Arg | −4.19 | 79.1 |
Tang1686 | Asp101Arg + Asp148Arg + Ser246Lys | −4.03 | 79.3 |
Tang1687 | Asp148Arg + Gly274Arg + Asp275Gln | −3.99 | 79.4 |
Tang1688 | Asn73Tyr + Asp148Arg + Gly274Arg | −3.89 | 88.3 |
Tang1689 | Ser75Lys + Ala248Phe + Gly274Arg | −3.75 | 77.5 |
Tang1690 | Asp148Arg + Thr245Ser + Gly274Arg | −3.68 | 78.9 |
Tang1691 | Asp148Arg + Ser246Lys + Gly274Arg | −3.63 | 73.7 |
Tang1692 | Ser75Lys + Ser246Lys + Gly274Arg | −3.45 | 75.0 |
Tang1693 | Asp148Arg + Ser246Lys + Phe247Pro | −3.36 | 73.5 |
Tang1694 | Asp148Arg + Phe273Lys + Gly274Arg | −3.36 | 73.6 |
Tang1695 | Asp148Arg + Tyr220Lys + Ser246Lys | −3.33 | 69.8 |
Tang1696 | Asp148Arg + Phe247Pro + Gly274Arg | −3.22 | 70.3 |
Tang1697 | Asp148Arg + Ser246Lys + Ala248Phe | −3.18 | 70.3 |
Tang1698 | Ser75Lys + Phe273Lys + Gly274Arg | −3.17 | 70.1 |
Tang1699 | Ser75Lys + Gly274Arg + Asp275Gln | −3.16 | 69.8 |