Fig. 6
From: Self-regulated 1-butanol production in Escherichia coli based on the endogenous fermentative control
Supplementation of complex nutrient is required to achieve efficient 1-butanol production. Strain ΔldhA ΔfrdBC ΔadhE Δpta transformed with the best combination (FRE ackA ::atoB-adhE2-crt-hbd, FRE adhE ::ter, FRE adhE ::fdh) was used in this study. Samples were taken after 24 h of anaerobic switch. M9 medium with and without 5 g/L of yeast extract (YE) was tested here for 1-butanol production in addition to the Terrific Broth (TB). GLC, glucose