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Table 1 Strains and plasmids used in this study

From: Self-regulated 1-butanol production in Escherichia coli based on the endogenous fermentative control

  Genotype Reference
Strain
BW25113 rrnB T14lacZ WJ16 hsdR514 ∆araBAD AH33rhaBAD LD78 [45]
XL-1 blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F’ proAB lacI q Z∆M15 Tn10 (Tetr)] Stratagene
JCL16 BW25113/F’ [traD36 proAB + lacI qZ∆M15 (Tetr)] [9]
JCL299 JCL16 ∆ldhAadhEfrdBCpta [9, 15]
Plasmid
pRW13 FRE ackA ::atoB (EC) adhE2 (CA) crt (CA) hbd (CA); ColE1 ori; Ampr This study
pRW14 FRE adhE ::atoB (EC) adhE2 (CA) crt (CA) hbd (CA); ColE1 ori; Ampr This study
pRW15 FRE frd ::atoB (EC) adhE2 (CA) crt (CA) hbd (CA); ColE1 ori; Ampr This study
pRW16 FRE ldhA ::atoB (EC) adhE2 (CA) crt (CA) hbd (CA); ColE1 ori; Ampr This study
pRW17 FRE ackA ::fdh (CB); pSC101 ori; Cmr This study
pRW18 FRE adhE ::fdh (CB); pSC101 ori; Cmr This study
pRW19 FRE frd ::fdh (CB); pSC101 ori; Cmr This study
pRW20 FRE ldhA ::fdh (CB); pSC101 ori; Cmr This study
pRW21 FRE ackA ::ter (TD); Cola ori; Kanr This study
pRW22 FRE adhE ::ter (TD); Cola ori; Kanr This study
pRW23 FRE frd ::ter (TD); Cola ori; Kanr This study
pRW24 FRE ldhA ::ter (TD); Cola ori; Kanr This study
pEL11 PLlacO1:: atoB (EC) adhE2 (CA) crt (CA) hbd (CA); ColE1 ori; Ampr [15]
pIM8 PLlacO1:: ter (TD); Cola ori; Kanr [15]
pCS138 PLlacO1:: fdh (CB); pSC101 ori; Cmr [15]
  1. Abbreviations in the parenthesis indicate source of the genes: EC, Escherichia coli; CA, Clostridium acetobutylicum; TD, Treponema denticola; CB, Candida boidinii