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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Utilization of xylose by engineered strains of Ashbya gossypii for the production of microbial oils

Fig. 2

Overexpression of the endogenous xylose utilization pathway in A. gossypii. a Pipeline of the construction of the GXX strain. The expression of the Cre recombinase enabled the loxP-KanMX-loxP marker to be eliminated (from the gray-colored strains) and subsequently reused. b Quantitative real-time PCR of the GRE3, XYL2, and XKS1 genes in the WT and GXX strains of A. gossypii. Relative transcription levels of the genes after 48 h of culture in MA2-rich medium. Transcription levels were normalized using the A. gossypii ACT1 gene as a reference. The results are the means of two independent experiments performed in duplicate and are expressed as a ratio of the cDNA abundances of the target genes with respect to the ACT1 mRNA levels. The inset represents the overexpression fold of the genes in the GXX strain with respect to the WT strain

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