Fig. 1

Transcript dynamics of the predicted DGAT genes in N. oceanica growing in nitrogen-replete (N+, dashed line) and nitrogen-depleted (N−, solid line) medium for 48 h analyzed by qPCR. Relative transcript levels are expressed as a fold change during 48 h after transfer to N-deprived conditions with respect to time 0. No significant differences in expression was found for DGAT1 and DGTT7–DGTT12 genes in response to N deprivation (a). The genes DGTT1 through DGTT6, which are up-regulated following N deprivation, (b) were chosen for further functional analysis. Each data point represents the average of three biological replicates. Each sample was analyzed in technical triplicates. Values represent mean ± SD (n = 3)