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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Nannochloropsis, a rich source of diacylglycerol acyltransferases for engineering of triacylglycerol content in different hosts

Fig. 5

Ectopic expression of NoDGTT5 in Arabidopsis leaves. a TAG content in wild-type (AtWT), control (AtEV), and NoDGTT5 over-expressing lines (At/D5a and At/D5b). b Changes in TAG composition between wild-type, control, and NoDGTT5 producing lines. c Representative confocal images of LDs labeled with BODIPY 493/503 (green) in the leaves of WT and transformed (At/D5b) Arabidopsis plants. Red color shows chlorophyll auto-fluorescence. Images are projections of Z-stacks of 28 optical sections taken 0.5 μm apart. ST stomata, Bars 25 μm. d LD counts in the leaves of WT, EV, and transformants expressing NoDGTT5 after labeling with BODIPY 493/503. Values in a, b and d represent averages and SD of three individual experiments and asterisks indicate P < 0.01

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