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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: Nannochloropsis, a rich source of diacylglycerol acyltransferases for engineering of triacylglycerol content in different hosts

Fig. 6

Constitutive expression of NoDGTT5 in seeds of WT and tag1-1 Arabidopsis plants. a Morphology (inserts) and ultrastructure of seeds before (AtWT and tag1-1) and after (At/D5a and tag1-1/D5a) expressing of NoDGTT5. N; nucleus, No; nucleolus, PB; protein body, asterisks indicate lipid droplets. Bars in the inserts = 250 μm; bars in the micrographs = 1 μm. b TAG contents in the seeds from WT plants, plants transformed with control vector (AtEV), WT plants over-expressing NoDGTT5 (At/D5a and At/D5b), mutant lines (tag1-1a and tag1-1b), and mutant lines complemented with NoDGTT5 gene (tag1-1/D5a and tag1-1/D5b). c, d Positional analysis of TAG composition between WT plants, control plants (EV) and lines over-expressing NoDGTT5 (At/D5a and At/D5b). c Content of sn-1/sn-3 moieties and d abundance of sn-2 moieties in respective TAG pools. e, f Positional analysis of TAG composition among mutant lines (tag1-1a and tag1-1b) and tag1-1 lines complemented with NoDGTT5 gene (tag1-1/D5a and tag1-1/D5b). In b–f averages of three replicates are shown with error bars indicating SD and with asterisks indicating P < 0.01

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