Fig. 12

Pex13-GFP signal in media with nitrogen limitation. a Cells were inoculated from a single flask after growth into 10 mL each of 4 different media [YNB with 2% glucose and 0.5% ammonium sulfate (w/v), YNB with no ammonium sulfate, YPD, and yeast extract-dextrose with no peptone added (Y-D)]. The cultures were grown shaking at 200 rpm for 16 h followed by imaging on a Leica LSM710 scanning confocal microscope. Upper and lower panels are replicate images for each condition above. b Images were quantified for integrated intensity using CellProfiler [135] with calcofluor signal to find cell area, and statistical analysis done by Statistica. All groups are statistically different except YNBnoN and YPD