Fig. 2

Utilization of vector pYL15 for expression of sfGFP fusion proteins. Vector pYL15 is linearized by digestion with SmaI. Coding sequences of interest are amplified with primers containing short 5′ and 3′ overhangs homologous to the pYL15 cut site. The vector and coding sequence are assembled and transformed into E. coli. The pYL15 vector contains Y. lipolytica centromere and origin of replication sequences derived from ARS68 [46] as well as leu2 for selection. Map is not drawn to scale to accommodate text