Fig. 7

Cell atlas prototrophs. The strains labeling 7 organelle compartments within cells in a prototrophic background. FEB130 was transformed with an endogenously targeted PCR products containing fragments to C-terminally tag organelle-specific proteins followed by a complimenting URA3 gene. Increased lipid droplet accumulation is accompanied by loss of peroxisomes, increase in hH1 signal, and other cellular changes tracked in a live cell without further processing steps. Strains were imaged at 16 h in YPD (t0) and after 20 h in Y-D media (t1). Top GFP channel; bottom GFP with transmission PMT overlay