Table 2 Ethanol yield of the engineered strains of G. thermoglucosidasius
Strains | Metabolite concentration (mM) after 24 h of fermentation | ||
|---|---|---|---|
Residual glucose | Ethanol | Ethanol yield (g/g) | |
NCIMB 11955 | 71.6 | 5.6 | 0.04 ± 0.01 |
LS001 (ΔpyrEΔldh) | 80.7 | 37.7 | 0.32 ± 0.07 |
LS003 (ΔpyrEΔldh pdh up) | 44.3 | 95.2 | 0.37 ± 0.04 |
LS004 (ΔpyrEΔldh pdh up Δpfl) | 81.5 | 18.6 | 0.16 ± 0.02 |
LS005 (ΔpyrEΔldh pdh up pfl −) | 81.1 | 25.5 | 0.22 ± 0.12 |
LS242 (Δldh pdh up Δpfl) | 77.6 | 25.1 | 0.19 ± 0.04 |
TM89 (ldh −) | 89.0 | 37.7 | 0.44 ± 0.01 |
TM003 (ΔpyrE ldh − pdh up) | 41.8 | 84.9 | 0.31 ± 0.01 |
TM004 (ΔpyrE ldh − pdh up Δpfl) | 80.5 | 18.1 | 0.15 ± 0.05 |
TM005 (ΔpyrE ldh − pfl −) | 77.5 | 31.9 | 0.24 ± 0.02 |
TM242 (ldh − pdh up pfl −) | 0 | 186.6 | 0.43 ± 0.01 |
LS003aa+(ΔpyrEΔldh pdh up Δpfl) | 0 | 182.7 | 0.42 ± 0.04 |
TM003aa+(ΔpyrE ldh − pdh up Δpfl) | 0 | 180.4 | 0.42 ± 0.01 |
TM242aa+(ldh − pdh up pfl −) | 0 | 180.2 | 0.42 ± 0.01 |