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Table 2 Ethanol yield of the engineered strains of G. thermoglucosidasius

From: Development and implementation of rapid metabolic engineering tools for chemical and fuel production in Geobacillus thermoglucosidasius NCIMB 11955

Strains Metabolite concentration (mM) after 24 h of fermentation
Residual glucose Ethanol Ethanol yield (g/g)
NCIMB 11955 71.6 5.6 0.04 ± 0.01
LS001 (ΔpyrEΔldh) 80.7 37.7 0.32 ± 0.07
LS003 (ΔpyrEΔldh pdh up) 44.3 95.2 0.37 ± 0.04
LS004 (ΔpyrEΔldh pdh up Δpfl) 81.5 18.6 0.16 ± 0.02
LS005 (ΔpyrEΔldh pdh up pfl ) 81.1 25.5 0.22 ± 0.12
LS242 (Δldh pdh up Δpfl) 77.6 25.1 0.19 ± 0.04
TM89 (ldh ) 89.0 37.7 0.44 ± 0.01
TM003 (ΔpyrE ldh pdh up) 41.8 84.9 0.31 ± 0.01
TM004 (ΔpyrE ldh pdh up Δpfl) 80.5 18.1 0.15 ± 0.05
TM005 (ΔpyrE ldh pfl ) 77.5 31.9 0.24 ± 0.02
TM242 (ldh pdh up pfl ) 0 186.6 0.43 ± 0.01
LS003aa+pyrEΔldh pdh up Δpfl) 0 182.7 0.42 ± 0.04
TM003aa+pyrE ldh pdh up Δpfl) 0 180.4 0.42 ± 0.01
TM242aa+(ldh pdh up pfl ) 0 180.2 0.42 ± 0.01
  1. aa+0.1 mM acetic acid added to culture medium
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