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Table 5 Plasmids used in this study

From: Development and implementation of rapid metabolic engineering tools for chemical and fuel production in Geobacillus thermoglucosidasius NCIMB 11955

Plasmid Relevant description Source
pTMO31 G. thermoglucosidasius shuttle vector, pMB1, pUB110, AmpR, KanR TMO
pNW33 N G. thermoglucosidasius shuttle vector, pMB1, pUB110, CmR BGSC
pUC31 G. thermoglucosidasius shuttle vector, pMB1, pUB110, KanSR TMO
pMTL85151 Clostridial modular shuttle vector, ColE1+tra, pIM13, CmR This study
pMTL62110 G. thermoglucosidasius modular shuttle vector, ColE1+tra, pUB110, KanR This study
pMTL63110 pMTL86551 with shorter pUB110 This study
pMTL61110 pMTL86551 with longer pUB110 This study
pMTL-LS1 Vector for generation of pyrE mutant via allelic exchange This study
pMTL-LS2 pyrE mutant correction vector This study
pMTL-LS3 Vector for integration of DNA at pyrE locus via allelic exchange This study
pMTL-LS3::pheB Vector for integrating pldh::pheB at the pyrE locus This study
pMTL-LS5 Complementation vector for pyrE mutant based on pyrE from G. kaustophilus This study
pMTL-LS6 Complementation vector for pyrE mutant based on pyrE from G. thermoleovorans This study
pMTL-LS5::pfl pfl in-frame deletion vector This study
pMTL-LS5::ldh ldh in-frame deletion vector This study
pMTL-LS5::ldhp:pdh pdh promoter replacement vector with ldh promoter from G. stearothermophilus This study
pMTL-LS5::pfl242 pfl deletion vector allowing disruption in the same context as that of TM242 This study
pMTL-LS5::aprt aprt in-frame deletion vector This study
pMTL-LS5::trpB trpB in-frame deletion vector This study
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