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Table 5 Plasmids used in this study

From: Development and implementation of rapid metabolic engineering tools for chemical and fuel production in Geobacillus thermoglucosidasius NCIMB 11955

Plasmid

Relevant description

Source

pTMO31

G. thermoglucosidasius shuttle vector, pMB1, pUB110, AmpR, KanR

TMO

pNW33 N

G. thermoglucosidasius shuttle vector, pMB1, pUB110, CmR

BGSC

pUC31

G. thermoglucosidasius shuttle vector, pMB1, pUB110, KanSR

TMO

pMTL85151

Clostridial modular shuttle vector, ColE1+tra, pIM13, CmR

This study

pMTL62110

G. thermoglucosidasius modular shuttle vector, ColE1+tra, pUB110, KanR

This study

pMTL63110

pMTL86551 with shorter pUB110

This study

pMTL61110

pMTL86551 with longer pUB110

This study

pMTL-LS1

Vector for generation of pyrE mutant via allelic exchange

This study

pMTL-LS2

pyrE mutant correction vector

This study

pMTL-LS3

Vector for integration of DNA at pyrE locus via allelic exchange

This study

pMTL-LS3::pheB

Vector for integrating pldh::pheB at the pyrE locus

This study

pMTL-LS5

Complementation vector for pyrE mutant based on pyrE from G. kaustophilus

This study

pMTL-LS6

Complementation vector for pyrE mutant based on pyrE from G. thermoleovorans

This study

pMTL-LS5::pfl

pfl in-frame deletion vector

This study

pMTL-LS5::ldh

ldh in-frame deletion vector

This study

pMTL-LS5::ldhp:pdh

pdh promoter replacement vector with ldh promoter from G. stearothermophilus

This study

pMTL-LS5::pfl242

pfl deletion vector allowing disruption in the same context as that of TM242

This study

pMTL-LS5::aprt

aprt in-frame deletion vector

This study

pMTL-LS5::trpB

trpB in-frame deletion vector

This study