Plasmid | Relevant description | Source |
---|---|---|
pTMO31 | G. thermoglucosidasius shuttle vector, pMB1, pUB110, AmpR, KanR | TMO |
pNW33Â N | G. thermoglucosidasius shuttle vector, pMB1, pUB110, CmR | BGSC |
pUC31 | G. thermoglucosidasius shuttle vector, pMB1, pUB110, KanSR | TMO |
pMTL85151 | Clostridial modular shuttle vector, ColE1+tra, pIM13, CmR | This study |
pMTL62110 | G. thermoglucosidasius modular shuttle vector, ColE1+tra, pUB110, KanR | This study |
pMTL63110 | pMTL86551 with shorter pUB110 | This study |
pMTL61110 | pMTL86551 with longer pUB110 | This study |
pMTL-LS1 | Vector for generation of pyrE mutant via allelic exchange | This study |
pMTL-LS2 | pyrE mutant correction vector | This study |
pMTL-LS3 | Vector for integration of DNA at pyrE locus via allelic exchange | This study |
pMTL-LS3::pheB | Vector for integrating pldh::pheB at the pyrE locus | This study |
pMTL-LS5 | Complementation vector for pyrE mutant based on pyrE from G. kaustophilus | This study |
pMTL-LS6 | Complementation vector for pyrE mutant based on pyrE from G. thermoleovorans | This study |
pMTL-LS5::pfl | pfl in-frame deletion vector | This study |
pMTL-LS5::ldh | ldh in-frame deletion vector | This study |
pMTL-LS5::ldhp:pdh | pdh promoter replacement vector with ldh promoter from G. stearothermophilus | This study |
pMTL-LS5::pfl242 | pfl deletion vector allowing disruption in the same context as that of TM242 | This study |
pMTL-LS5::aprt | aprt in-frame deletion vector | This study |
pMTL-LS5::trpB | trpB in-frame deletion vector | This study |