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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Development of a genome-editing CRISPR/Cas9 system in thermophilic fungal Myceliophthora species and its application to hyper-cellulase production strain engineering

Fig. 2

Phenotypic analysis of constitutive Cas9-expressing strains. a Fluorescence microscopic assessment of Cas9-gfp localization in M. thermophila. The nuclei were stained with 4′,6-diamidino-2-phenylindole. Each scale bar represents 10 µm. b Colony growth and sporulation of Cas9OE and wild-type (WT) strains on minimal medium plates after 4 days of culture

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