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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Development of a genome-editing CRISPR/Cas9 system in thermophilic fungal Myceliophthora species and its application to hyper-cellulase production strain engineering

Fig. 5

Phenotypic analysis of Δcre-1 and wild-type strains. a Colonies of Δcre-1 (ΔMtcre-1 and ΔMhcre-1) and wild-type strains (MtWT and MhWT) of M. thermophila and M. heterothallica on minimal medium plates after 2 days. b Sodium dodecylsulfate-polyacrylamide gel electrophoresis of secreted protein of Δcre-1 and wild-type strains after 4 days culture in 2% Avicel medium. c–f Assays for protein concentration and xylanase, endoglucanase, and exoglucanase activities of Δcre-1 and wild-type strains in 2% Avicel inducing medium after 3 and 4 days culture. Bars marked by asterisks in each group differ significantly from unmarked ones (Tukey’s HSD, p < 0.001). Error bars represent SD from three replicates

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