Fig. 2
Growth performances of the selected segregants from each generation of backcrossing. One segregant of the cross between strains JL1 and CEN.PK113-7D, exerting the best growth performance on glycerol, was selected and denominated F1. This strain was backcrossed with strain CEN.PK113-7D for two successive rounds of backcrosses, yielding strains F2 and F3 both selected for the highest growth rate on glycerol among the respective progeny. Strain F3 was sent for whole-genome resequencing for further analysis. Data from one representative experiment out of three independent biological replicates are shown. Average values and standard deviations of µmax are shown in the box