Table 1 Yeast strains used in this study

CEN.PK113-1A

MATα (prototrophic)

Euroscarf

PW-1 (CEN.PK113-1A)

MATα (prototrophic) evolved for growth on glycerol

This study

PW-2 (CEN.PK113-1A)

MATα (prototrophic) evolved for growth on glycerol

This study

CBS 6412-13A

MATa haploid segregant of CBS 6412

[20]

CEN.PK113-1A GUT1 _CBS UBR2 _CBS SSK1 _CBS

MATα; gut1::GUT1 _{CBS 6412-13A} ubr2::UBR2 _{CBS 6412-13A} ssk1::SSK1 _{CBS 6412-13A}

[22]

CEN.PK113-7D

MATa (prototrophic)

[5]

JL1 (CEN.PK113-7D)

MATa (prototrophic); evolved for growth on glycerol

[5]

JL1-2 (CEN.PK113-7D)

MATα (prototrophic); mating type switched from JL1

This study

CEN.PK113-7D GUT1 _JL1

MATa; gut1::GUT1 _JL1 allelic replacement analysis of GUT1 _JL1

This study

CEN.PK113-7D GUT1 _PW-1

MATa; gut1::GUT1 _PW-1 allelic replacement analysis of GUT1 _PW-1

This study

CEN.PK113-7D GUT1 _PW-2

MATa; gut1::GUT1 _PW-2 allelic replacement analysis of GUT1 _PW-2

This study

CEN.PK113-7D GUT1 _CBS

MATa; gut1::GUT1 _CBS allelic replacement analysis of GUT1 _{CBS 6412-13A}

This study

CEN.PK113-7D UBR2 _JL1

MATa; ubr2::UBR2 _JL1 allelic replacement analysis of UBR2 _JL1

This study

CEN.PK113-7D UBR2 _PW-1

MATa; ubr2::UBR2 _PW-1 allelic replacement analysis of UBR2 _PW-1

This study

CEN.PK113-7D UBR2 _PW-2

MATa; ubr2::UBR2 _PW-2 allelic replacement analysis of UBR2 _PW-2

This study

CEN.PK113-7D UBR2 _CBS

MATa; ubr2::UBR2 _CBS allelic replacement analysis of UBR2 _{CBS 6412-13A}

This study

CEN.PK113-7D GUT1 _JL1 UBR2 _JL1

MATa; gut1::GUT1 _JL1 ubr2::UBR2 _JL1 allelic replacement analysis of GUT1 _JL1 and UBR2 _JL1

This study

CEN.PK113-7D GUT1 _PW-1 UBR2 _PW-1

MATa; gut1::GUT1 _PW-1 ubr2::UBR2 _PW-1 allelic replacement analysis of GUT1 _PW-1 and UBR2 _PW-1

This study

CEN.PK113-7D GUT1 _PW-2 UBR2 _PW-2

MATa; gut1::GUT1 _PW-2 ubr2::UBR2 _PW-2 allelic replacement analysis of GUT1 _PW-2 and UBR2 _PW-2

This study

CEN.PK113-7D GUT1 _CBS UBR2 _CBS

MATa; gut1::GUT1 _CBS ubr2::UBR2 _CBS allelic replacement analysis of GUT1 _{CBS 6412-13A} and UBR2 _{CBS 6412-13A}

This study