Fig. 3

Glycome profiles obtained from successive extractions of the solid fractions remaining after reactions with corn stover (CS) indicated at the bottom of the figure. From left to right, the solid fractions are from samples of untreated CS (not boiled and boiled), AFEX-CS (not boiled and boiled), and AFEX-CS reacted with either CMX00_3a, XynY or XynA. Enzyme digestion reactions were incubated for 24 h at 55 °C and included 1 g of substrate in a 10-mL reaction with either no enzyme, 0.3 µmol of CMX00_3a, 0.1 µmol of XynY, or 0.2 µmol of XynA as indicated. All enzyme reactions were boiled to stop the enzyme reaction. The total carbohydrate extracted at each step of the glycome profiling workup is shown as the bar graph at the top of the figure. Each extract was loaded at 300 ng glucose equivalents/well on the ELISA plates. The glycan binding specificities of different antibody clades are shown at the right [45], with the following abbreviations: XG xyloglucan, HG homogalacturonan, RG rhamnogalacturonan, and AG arabinogalactan. Further information on the binding specificities of the antibodies is provided in the Additional file 1. The color scheme of the heatmaps depicts mAb binding strengths with dark blue representing no binding, red intermediate, and bright yellow strongest binding