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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling

Fig. 4

Glycome profiles obtained from successive extractions of the solid fractions remaining after reactions with switchgrass (SG) indicated at the bottom of the figure. From left to right, the solid fractions are from samples of untreated SG (not boiled and boiled), AFEX-SG (not boiled and boiled), and AFEX-SG reacted with either CMX00_3a, XynY or XynA. Enzyme digestion reactions were carried out as in Fig. 3. Enzyme reactions were boiled to inactivate the enzyme. The total carbohydrate extracted at each step of the glycome profiling workup is shown as the bar graph at the top of the figure. Each extract was loaded at 300 ng glucose equivalents/well on the ELISA plates. The glycan binding specificities of different antibody clades are shown at the right [45], with the following abbreviations: XG xyloglucan, HG homogalacturonan, RG rhamnogalacturonan, and AG arabinogalactan. Further information on the binding specificities of the antibodies is provided in the Additional file 1: Table S1. The color scheme of the heatmaps depicts mAb binding strengths with dark blue representing no binding, red intermediate, and bright yellow strongest binding

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