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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling

Fig. 6

Oxime-NIMS analysis of enzyme activity (µmol of reducing sugar released per µmol of pure enzyme per hour at 55 °C) for different classes of reducing sugar products obtained from enzymatic hydrolysis of AFEX-corn stover or AFEX-switchgrass. Hexose products are derived from cellulose, while pentose and mixed pentose/hexose products (e.g., for mixed products, Xyl–Glc, Xyl–Xyl-Glc, Glc–Glc–Xyl, Xyl–Xyl–Xyl–Glc, Xyl–Rha–GalA–Xyl) are from various hemicellulose polysaccharides. Tables 3 and 4 list the concentrations of individual soluble oligosaccharides detected by NIMS in each of these categories. The color bars depict the different enzymes used: CMX00_3a (purple); XynY (orange); XynA (green)

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