Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling

Table 1 Soluble oligosaccharide products detected by oxime-NIMS from hydrolysis of purified polysaccharides

	P1^a	P2	P3	P4	P5	Total	Average	Stdev
Oat spelt xylan
CMX00_CBM3a	2	27	43	28	14	113	125	13
	2	28	49	31	14	124
	2	29	48	40	19	139
XynY	225	403	114	68	15	826	855	37
	236	455	110	83	13	896
	216	420	104	88	13	842
XynA	88	277	2	5	118	488	468	25
	80	285	4	4	104	477
	80	260	3	5	93	440

	P1^b	P2	P3	P4	P5	Total	Average	Stdev
Arabinoxylan
CMX00_CBM3a	1	1	0	0	0	1	1	0
	1	0	0	0	0	1
	1	0	0	0	0	1
XynY	34	87	20	26	21	188	200	16
	39	102	24	29	24	219
	36	90	20	25	22	193
XynA	16	30	0	3	11	61	62	2
	17	34	0	3	11	65
	16	32	0	2	11	61

Additional abbreviations used: P1 pentose (i.e., likely xylose), P2 pentobiose, P3 pentotriose, P4 pentotetraose, P5 pentopentose, with ~70:15:10 composition of xylose:glucose:arabinose present. No mass signatures for hexose sugars were identified
^aActivity reported as µmol of reducing sugar released per hour per µmol of purified enzyme determined by oxime-NIMS as described in “Methods” section
^b P1 pentose (xylose and arabinose will give rise to the same mass signature in oxime-NIMS; with a 38:62 composition of arabinose and xylose and less than 1% of glucose, galactose, and mannose present; no mass signatures for hexose sugars were identified), P2 pentobiose, P3 pentotriose, P4 pentotetraose, P5 pentopentose

ISSN: 2731-3654