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Table 1 Soluble oligosaccharide products detected by oxime-NIMS from hydrolysis of purified polysaccharides

From: Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling

  P1a P2 P3 P4 P5 Total Average Stdev
Oat spelt xylan
 CMX00_CBM3a 2 27 43 28 14 113 125 13
2 28 49 31 14 124   
2 29 48 40 19 139   
 XynY 225 403 114 68 15 826 855 37
236 455 110 83 13 896   
216 420 104 88 13 842   
 XynA 88 277 2 5 118 488 468 25
80 285 4 4 104 477   
80 260 3 5 93 440   
  1. Additional abbreviations used: P1 pentose (i.e., likely xylose), P2 pentobiose, P3 pentotriose, P4 pentotetraose, P5 pentopentose, with ~70:15:10 composition of xylose:glucose:arabinose present. No mass signatures for hexose sugars were identified
  2. aActivity reported as µmol of reducing sugar released per hour per µmol of purified enzyme determined by oxime-NIMS as described in “Methods” section
  3. b P1 pentose (xylose and arabinose will give rise to the same mass signature in oxime-NIMS; with a 38:62 composition of arabinose and xylose and less than 1% of glucose, galactose, and mannose present; no mass signatures for hexose sugars were identified), P2 pentobiose, P3 pentotriose, P4 pentotetraose, P5 pentopentose
  P1b P2 P3 P4 P5 Total Average Stdev
Arabinoxylan
 CMX00_CBM3a 1 1 0 0 0 1 1 0
1 0 0 0 0 1   
1 0 0 0 0 1   
 XynY 34 87 20 26 21 188 200 16
39 102 24 29 24 219   
36 90 20 25 22 193   
 XynA 16 30 0 3 11 61 62 2
17 34 0 3 11 65   
16 32 0 2 11 61   
  1. Additional abbreviations used: P1 pentose (i.e., likely xylose), P2 pentobiose, P3 pentotriose, P4 pentotetraose, P5 pentopentose, with ~70:15:10 composition of xylose:glucose:arabinose present. No mass signatures for hexose sugars were identified
  2. aActivity reported as µmol of reducing sugar released per hour per µmol of purified enzyme determined by oxime-NIMS as described in “Methods” section
  3. b P1 pentose (xylose and arabinose will give rise to the same mass signature in oxime-NIMS; with a 38:62 composition of arabinose and xylose and less than 1% of glucose, galactose, and mannose present; no mass signatures for hexose sugars were identified), P2 pentobiose, P3 pentotriose, P4 pentotetraose, P5 pentopentose