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Table 2 Amount of carbohydrate extracted during glycome profiling per gram of cell wall

From: Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling

 

Untreated

Boiled

AFEX

AFEX, boiled

CMX00

XynY

XynA

Corn stover

 Oxalate

2.3 ± 0.0

5.9 ± 0.0

5.0 ± 1.1

8.6 ± 1.4

4.1 ± 0.1

3.9 ± 0.1

9.6 ± 0.9

 Carbonate

3.2 ± 0.2

4.4 ± 0.1

5.1 ± 0.5

4.6 ± 0.4

4.4 ± 0.8

4.0 ± 2.0

9.3 ± 0.6

 1 M KOH

81.4 ± 1.5

61.4 ± 1.9

48.7 ± 1.8

46.4 ± 0.2

31.9 ± 0.9

21.1 ± 0.1

20.2 ± 1.4

 4 M KOH

44.5 ± 2.1

26.3 ± 1.4

37.4 ± 1.8

32.7 ± 7.9

21.6 ± 2.6

11.1 ± 1.7

18.7 ± 0.2

 Chlorite

7.0 ± 1.3

5.8 ± 0.4

3.6 ± 0.4

5.8 ± 1.3

2.2 ± 0.1

3.4 ± 0.6

2.9 ± 0.1

 4 M KOHPC

9.4 ± 0.8

7.1 ± 2.9

7.8 ± 1.0

8.6 ± 2.8

4.2 ± 0.7

6.8 ± 3.5

3.2 ± 1.1

 Total solida

147.9

111.0

107.5

106.8

68.4

50.3

63.9

 Solubleb

1.5

1.5

1.5

1.5

44.6

65.5

58.9

 Totalc

149.4

112.5

109.0

108.3

113.1

115.8

122.8

Switchgrass

 Oxalate

1.7 ± 0.5

1.8 ± 0.7

3.4 ± 0.5

5.5d

3.2 ± 0.0

1.5 ± 0.6

6.5 ± 1.1

 Carbonate

2.5 ± 1.2

2.2 ± 0.3

3.4 ± 0.7

3.2d

2.6 ± 0.1

3.6 ± 1.6

4.8 ± 0.2

 1 M KOH

58.4 ± 13.1

63.9 ± 13.3

30.6 ± 10.0

64.4d

33.8 ± 13.5

13.9 ± 1.0

30.6 ± 7.6

 4 M KOH

20. ± 5.1

37.9 ± 9.0

14.0 ± 8.3

19.8d

13.7 ± 2.4

9.0 ± 2.0

8.8 ± 4.2

 Chlorite

7.2 ± 0.4

7.0 ± 0.7

8.3 ± 1.3

6.8d

5.7 ± 0.7

9.3 ± 3.3

7.1 ± 1.1

 4 M KOHPC

10.8 ± 12.0

29.8 ± 5.7

18.5 ± 1.8

23.8d

15.6 ± 1.4

8.1 ± 0.5

14.2 ± .1

 Total solida

100.5

142.5

78.2

123.3d

74.5

45.4

72.1

 Solubleb

0.6

0.6

0.6

0.6d

46.3

69.8

70.0

 Totalc

101.1

143.1

78.8

123.9d

120.9

115.2

142.1

  1. aReducing sugar equivalents extracted from the solid fraction and quantified as mg quantities as reported elsewhere [46, 47]. Standard deviation calculated from two experimental replicates carried through the entire analysis method
  2. bReducing sugar equivalents present in the soluble fraction determined by oxime-NIMS (mg)
  3. cSum of solid and soluble fractions
  4. dFor boiled, AFEX-pretreated switchgrass, only single reactions were performed