Skip to main content

Advertisement

Table 2 Amount of carbohydrate extracted during glycome profiling per gram of cell wall

From: Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling

  Untreated Boiled AFEX AFEX, boiled CMX00 XynY XynA
Corn stover
 Oxalate 2.3 ± 0.0 5.9 ± 0.0 5.0 ± 1.1 8.6 ± 1.4 4.1 ± 0.1 3.9 ± 0.1 9.6 ± 0.9
 Carbonate 3.2 ± 0.2 4.4 ± 0.1 5.1 ± 0.5 4.6 ± 0.4 4.4 ± 0.8 4.0 ± 2.0 9.3 ± 0.6
 1 M KOH 81.4 ± 1.5 61.4 ± 1.9 48.7 ± 1.8 46.4 ± 0.2 31.9 ± 0.9 21.1 ± 0.1 20.2 ± 1.4
 4 M KOH 44.5 ± 2.1 26.3 ± 1.4 37.4 ± 1.8 32.7 ± 7.9 21.6 ± 2.6 11.1 ± 1.7 18.7 ± 0.2
 Chlorite 7.0 ± 1.3 5.8 ± 0.4 3.6 ± 0.4 5.8 ± 1.3 2.2 ± 0.1 3.4 ± 0.6 2.9 ± 0.1
 4 M KOHPC 9.4 ± 0.8 7.1 ± 2.9 7.8 ± 1.0 8.6 ± 2.8 4.2 ± 0.7 6.8 ± 3.5 3.2 ± 1.1
 Total solida 147.9 111.0 107.5 106.8 68.4 50.3 63.9
 Solubleb 1.5 1.5 1.5 1.5 44.6 65.5 58.9
 Totalc 149.4 112.5 109.0 108.3 113.1 115.8 122.8
Switchgrass
 Oxalate 1.7 ± 0.5 1.8 ± 0.7 3.4 ± 0.5 5.5d 3.2 ± 0.0 1.5 ± 0.6 6.5 ± 1.1
 Carbonate 2.5 ± 1.2 2.2 ± 0.3 3.4 ± 0.7 3.2d 2.6 ± 0.1 3.6 ± 1.6 4.8 ± 0.2
 1 M KOH 58.4 ± 13.1 63.9 ± 13.3 30.6 ± 10.0 64.4d 33.8 ± 13.5 13.9 ± 1.0 30.6 ± 7.6
 4 M KOH 20. ± 5.1 37.9 ± 9.0 14.0 ± 8.3 19.8d 13.7 ± 2.4 9.0 ± 2.0 8.8 ± 4.2
 Chlorite 7.2 ± 0.4 7.0 ± 0.7 8.3 ± 1.3 6.8d 5.7 ± 0.7 9.3 ± 3.3 7.1 ± 1.1
 4 M KOHPC 10.8 ± 12.0 29.8 ± 5.7 18.5 ± 1.8 23.8d 15.6 ± 1.4 8.1 ± 0.5 14.2 ± .1
 Total solida 100.5 142.5 78.2 123.3d 74.5 45.4 72.1
 Solubleb 0.6 0.6 0.6 0.6d 46.3 69.8 70.0
 Totalc 101.1 143.1 78.8 123.9d 120.9 115.2 142.1
  1. aReducing sugar equivalents extracted from the solid fraction and quantified as mg quantities as reported elsewhere [46, 47]. Standard deviation calculated from two experimental replicates carried through the entire analysis method
  2. bReducing sugar equivalents present in the soluble fraction determined by oxime-NIMS (mg)
  3. cSum of solid and soluble fractions
  4. dFor boiled, AFEX-pretreated switchgrass, only single reactions were performed