Table 4 Enzyme activities for AFEX-SG hydrolysis measured by oxime-NIMS
G1 (mM) | G2 (mM) | G3 (mM) | G4 (mM) | Total (mM) | Average (mM) | Stdev | |||
|---|---|---|---|---|---|---|---|---|---|
No enzyme | 0.2 | 0.0 | 0.0 | 0.0 | 0.58 | – | – | ||
CMX00_3a | 4.6 | 5.8 | 0.2 | 0.1 | 8.54 | ||||
4.5 | 5.0 | 0.1 | 0.0 | 7.67 | 10.2 | 0.8 | |||
XynY | 0.0 | 1.3 | 1.5 | 0.2 | 0.76 | ||||
0.0 | 1.0 | 1.0 | 0.2 | 0.54 | 2.6 | 0.6 | |||
XynA | 0.0 | 0.0 | 0.0 | 0.0 | 0.02 | ||||
0.0 | 0.0 | 0.0 | 0.0 | 0.00 | 0.0 | 0.0 |
P1 (mM) | P2 (mM) | P3 (mM) | P4 (mM) | P5 (mM) | P6 (mM) | Total (mM) | Average (mM) | Stdev | |
|---|---|---|---|---|---|---|---|---|---|
No enzyme | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | – | – |
CMX00_3a | 3.2 | 14.5 | 17.3 | 6.7 | 1.6 | 0.3 | 43.6 | ||
3.1 | 15.3 | 17.0 | 6.6 | 1.1 | 0.2 | 43.3 | 43.4 | 0.2 | |
XynY | 94.5 | 127.9 | 40.5 | 10.5 | 1.1 | 0.1 | 274.6 | ||
93.1 | 143.3 | 40.1 | 12.0 | 0.9 | 0.0 | 289.5 | 282.0 | 10.5 | |
XynA | 36.7 | 94.5 | 0.3 | 2.2 | 10.7 | 1.0 | 145.3 | ||
39.3 | 95.0 | 0.1 | 1.5 | 11.0 | 0.3 | 147.3 | 146.3 | 1.4 |
Xa (mM) | XG (mM) | X2-G (mM) | X3-G (mM) | Xyl-Rha-GalA-Xyl (mM) | Total (mM) | Average (mM) | Stdev | ||
|---|---|---|---|---|---|---|---|---|---|
No enzyme | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | – | – | |
CMX00_3a | 2.9 | 0.1 | 1.4 | 0.2 | 0.6 | 5.2 | |||
1.4 | 0.1 | 1.1 | 0.2 | 0.6 | 3.4 | 4.3 | 1.3 | ||
XynY | 0.4 | 0.0 | 0.2 | 0.1 | 2.6 | 3.3 | |||
0.4 | 0.0 | 0.2 | 0.1 | 2.5 | 3.1 | 3.2 | 0.1 | ||
XynA | 0.4 | 0.0 | 0.0 | 0.0 | 0.1 | 0.5 | |||
0.2 | 0.0 | 0.0 | 0.0 | 0.1 | 0.3 | 0.4 | 0.1 |