Fig. 5

Distinct roles of GLT-1 and HGT-1/-2 in plant cell wall-degrading enzyme production. a Secreted protein production of Avicel cultures of the WT, Δglt-1, Δ2hgt, Δ2hgt;Δglt-1, and a series of Δ2hgt::glucose transporter strains. The means and standard deviations of triplicate are shown. b Subcellular localization of HGT-1, HGT-2, and GLT-1 in Neurospora crassa. A transformant harboring pMF272-eGFP was the negative control. The scale bar corresponds to 10 µm. c Cellulase activity (CMCase) and xylanase activity per gram dried cell weight of Avicel culture supernatants at 7 days. Relative activities vs. the WT are color-coded. The statistical significance of mutant values compared with the WT was determined using a two-tailed Student’s t-test; *P < 0.05, **P < 0.01