Fig. 6

Simultaneous deletion of HGT-1/-2 leads to derepression of genes encoding lignocellulases and relief of catabolite repression. Relative expression levels of a two transcription factors (cre-1 and clr-2) and b lignocellulases (gh6-1, gh5-1, gh3-4, and gh11-2) in Δglt-1, Δ2hgt, and Δ2hgt;Δglt-1 mutants vs. the WT after 2–4 days growth on Avicel. Average values and standard deviations of two biological replicates/two technical replicates are shown. c Hierarchical clustering of RPKM values for 629 differentially expressed genes in Δ2hgt vs. the WT. The gene counts of each group are shown in brackets. d Secreted protein production of Avicel plus 0.1% 2-DG cultures of the WT, Δ2hgt, and Δcre-1. The means and standard deviations of triplicate are shown. e Growth phenotypes of the cultures in panel D