Fig. 6

Role of T-6b during fatty acid metabolism. a Expression levels of WRI1 and DGAT1 genes in siliques at different developmental stages. b Thin layer chromatography analysis of lipids isolated from mature seeds. Loading volume equals 2.5 mg mature seeds for both Col-0 and AtG6b. c Total TAG quantities in mature seeds of wild-type and AtG6b were determined by gas chromatography (up panel). Relative intensity of lipid species was calculated by ImagJ (below panel). *P < 0.05 and **P < 0.01 by Student’s t-test. Error bars indicate the standard deviation and n = 3