Fig. 7

Lipid content and fatty acid composition analysis in both embryo and endosperm. a The dissected endosperm/seed coat and embryo of mature Arabidopsis seeds after soften in water for 6 h at 4 °C (left panel). TLC separation of lipids isolated from endosperm (extraction volumes equivalent to 0.6 mg seed mass for both Col-0 and AtG6b) and embryo (extraction volumes equivalent to 0.3 mg seed mass for both Col-0 and AtG6b). TAG is indicated by an arrow. b TAG quantities in mature endosperm and embryo of wild-type and AtG6b were determined by gas chromatography. Error bars indicate the standard deviation and n = 3. **P < 0.01 by Student’s t-test. c Percentage of various fatty acid compositions in the whole seed, endosperm, and embryo, respectively. Error bars indicate the standard deviation and n = 3