Table 1 Strains and plasmids used in this study

Strains

 Mach1-T1R

F⁻ φ80(lacZ)ΔM15 ΔlacX74 hsdR(r_K-mK +) ΔrecA1398 endA1 tonA

Invitrogen

 W3110

F- λ- rph-1 IN(rrnD, rrnE)1

ATCC 27325

 DHK1

W3100 rpsL (A128G)/pET-cadA

This study

 DHK2

DHK1 P_galR-UTR_galR-galR-P_lysA-UTR_lysA-lysA:: P_BBa__J23100-SynUTR_asd-asd-P_BBa__J23100-SynUTR_dapA-dapA ^fbr-P_BBa__J23100-SynUTR_dapB-dapB-P_BBa__J23100-SynUTR_ddh-ddh-P_BBa__J23100-SynUTR_lysA-lysA P_lysC-UTR_lysC-lysC:: P_BBa__J23100-SynUTR_lysC-lysC ^fbr

This study

 DHK3

DHK2 galETKM:: P_BBa__J23100-SynUTR_galE-galE-_PBBa__J23100-SynUTR_galT-galT-_PBBa__J23100-SynUTR_galK-galK-_PBBa__J23100-SynUTR_galM- galM P_galP-UTR_galP::P_{BBa_J23100}-SynUTR_galP P_pgm-UTR_pgm::P_{BBa_J23100}-SynUTR_pgm

This study

 DHK4

DHK3 ΔspeE ΔspeG ΔygjG ΔpuuPA

This study

Plasmids

 pKD46

Red recombinase expression vector, Amp^R

[43]

 pCP20

FLP expression vector, Amp^R, Cm^R

[43]

 pETduet

Expression vector, ColE1 ori, Amp^R

Novagen

 pACYCduet

Expression vector, p15A ori, Cm^R

Novagen

 pACYC-Lys

p15A ori, Cm^R, P_BBa__J23100-SynUTR_asd-asd- P_BBa__J23100-SynUTR_dapA-dapA- P_BBa__J23100-SynUTR_dapB-dapB ^fbr- P_BBa__J23100-SynUTR_ddh-ddh- P_BBa__J23100-SynUTR_lysA-lysA

This study

 pET-cadA

ColE1 ori, Amp^R, P_BBa__J23100-SynUTR(v3)_cadA–cadA

This study

 pACYC-galO

p15A ori, Cm^R, FRT-Kan^R-FRT-P_{BBa_J23100}-SynUTR_galE-galE-P_{BBa_J23100}-SynUTR_galT-galT -P_{BBa_J23100}-SynUTR_galK-galK -P_{BBa_J23100}-SynUTR_galM-galM

[15]