Table 2 Key enzymes relevant to formate pathways identified by blasting the U. isabellina genome
From: Exploring eukaryotic formate metabolisms to enhance microbial growth and lipid accumulation
Name | Reaction |
|---|---|
NAD-dependent formate dehydrogenase (FDH, EC 1.2.1.1) | Formate + NAD+ ↔ CO2 + NADH + H+ |
Formate tetrahydrofolate ligase (EC6.3.4.3) | ATP + formate + tetrahydrofolate ↔ ADP + phosphate + 10-formyltetrahydrofolate |
Methenyltetrahydrofolate cyclohydrolase/dehydrogenase (EC3.5.4.9 and 1.5.1.5/15) | 5,10-Methenyltetrahydrofolate + H2O ↔ 10-formyltetrahydrofolate |
Glycine dehydrogenase (EC1.4.1.10) | Glycine + H2O + NAD + ↔ glyoxylate + NH3 + NADH + H+ |
Aminomethyltransferase (EC2.1.2.10) | Glycine + tetrahydrofolate + NAD+ ↔ 5,10-methylene-tetrahydrofolate + ammonium + CO2 + NADH |
Dihydrolipoyl dehydrogenase (EC1.8.1.4) | Protein N6-(dihydrolipoyl)lysine + NAD+ ↔ protein N6-(lipoyl)lysine + NADH + H+ |
Serine hydroxymethyltransferase (EC2.1.2.1) | 5,10-Methylenetetrahydrofolate + glycine + H2O ↔ tetrahydrofolate + l-serine |
Serine deaminase (EC4.3.1.17) | l-serine ↔ pyruvate + NH3 |