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Table 2 Key enzymes relevant to formate pathways identified by blasting the U. isabellina genome

From: Exploring eukaryotic formate metabolisms to enhance microbial growth and lipid accumulation

Name Reaction
NAD-dependent formate dehydrogenase (FDH, EC 1.2.1.1) Formate + NAD+ ↔ CO2 + NADH + H+
Formate tetrahydrofolate ligase (EC6.3.4.3) ATP + formate + tetrahydrofolate ↔ ADP + phosphate + 10-formyltetrahydrofolate
Methenyltetrahydrofolate cyclohydrolase/dehydrogenase (EC3.5.4.9 and 1.5.1.5/15) 5,10-Methenyltetrahydrofolate + H2O ↔ 10-formyltetrahydrofolate
Glycine dehydrogenase (EC1.4.1.10) Glycine + H2O + NAD + ↔ glyoxylate + NH3 + NADH + H+
Aminomethyltransferase (EC2.1.2.10) Glycine + tetrahydrofolate + NAD+ ↔ 5,10-methylene-tetrahydrofolate + ammonium + CO2 + NADH
Dihydrolipoyl dehydrogenase (EC1.8.1.4) Protein N6-(dihydrolipoyl)lysine + NAD+ ↔ protein N6-(lipoyl)lysine + NADH + H+
Serine hydroxymethyltransferase (EC2.1.2.1) 5,10-Methylenetetrahydrofolate + glycine + H2O ↔ tetrahydrofolate + l-serine
Serine deaminase (EC4.3.1.17) l-serine ↔ pyruvate + NH3