Fig. 1
From: Efficient estimation of the maximum metabolic productivity of batch systems
Method overview. A flow chart for how productivity–yield surfaces are calculated. Stoichiometric metabolic models (a) are generated from literature sources or genome annotations and are used to calculate the extreme elementary modes of the system (c). Literature data from growth experiments (b) are used together with stoichiometric models to fit dynamic expressions for substrate uptake kinetics and cellular growth (d). Bounds on substrate uptake are used with the set of EFMs in dynamic optimization (e) in order to calculate optimal flux profiles. This procedure is repeated to maximize product yield for a number of specified productivity constraints (e), generating the surface of maximum yield as a function of desired productivity, (f)