Fig. 4

Cel7A protein purification and enzyme activity of the purified protein on pretreated corn stover (PCS) as a substrate. a Coomassie-stained SDS-PAGE gel showing a single band of ~53 kDa purified Cel7A protein. b A parallel SDS-PAGE gel was used to identify the purified proteins by western blotting using anti-Cel7A antibody. c Cellulase enzyme activity of Cel7A proteins obtained from expression of the two different versions of the 2A cassette (i.e., G2C and C2G), compared to purified P. funiculosum Cel7A, shows similar conversion pattern. M molecular weight marker, Lanes 1 and 2 represent purified Cel7A proteins from G2C and C2G cassettes, respectively. Lane C represents purified P. funiculosum Cel7A