Biotechnology for Biofuels and Bioproducts

Table 1 Gene expression detection by fluorescence and enzyme assay of cell-free extract in recombinant strains

From: Enhancement of furan aldehydes conversion in Zymomonas mobilis by elevating dehydrogenase activity and cofactor regeneration

	Fluorescent detection^a	Activity (mU/mg crude protein)
	Fluorescent detection^a	Furfural + NADPH	HMF + NADPH	Furfural + NADH	HMF + NADH
ZM4(pHW20a-gfp) (Control)	+	21.15 ± 0.81	11.12 ± 0.45	110.87 ± 3.82	100.83 ± 2.73
ZM4(pHW20a-ZMO0062)	+	24.53 ± 0.99	14.82 ± 0.05	175.22 ± 9.91	135.51 ± 6.61
ZM4(pHW20a-ZMO1696)	+	29.40 ± 0.85	13.62 ± 0.25	134.00 ± 2.83	105.14 ± 1.62
ZM4(pHW20a-ZMO1722)	+	23.87 ± 0.87	12.24 ± 0.00	104.51 ± 2.69	99.40 ± 6.91
ZM4(pHW20a-ZMO1771)	+	32.31 ± 1.46	18.22 ± 0.45	102.97 ± 2.65	100.85 ± 3.89
ZM4(pHW20a-ZMO1993)	+	22.16 ± 0.00	11.62 ± 0.00	115.46 ± 0.00	101.64 ± 4.96
ZM4(pHW20a-ZMO0976)	+	62.24 ± 7.65	15.97 ± 0.38	133.27 ± 6.70	110.50 ± 3.19
ZM4(pHW20a-ZMO1344)	+	21.64 ± 0.00	12.34 ± 0.45	108.83 ± 2.80	94.19 ± 4.76
ZM4(pHW20a-ZMO1673)	+	22.31 ± 0.50	13.25 ± 0.30	116.88 ± 5.01	106.93 ± 2.39
ZM4(pHW20a-ZMO1773)	+	21.66 ± 0.49	11.89 ± 0.29	134.06 ± 4.86	107.71 ± 3.24
ZM4(pHW20a-ZMO1984)	+	20.46 ± 0.83	11.37 ± 0.46	99.31 ± 0.00	86.39 ± 2.84

Standard deviations were derived from at least two independent determinations
^a+: fluorescence can be detected under fluorescence microscope; −: no fluorescence under fluorescence microscope

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ISSN: 2731-3654

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