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Table 4 Cell growth, glucose consumption, ethanol formation, and furan aldehydes conversion of co-expression recombinant strains

From: Enhancement of furan aldehydes conversion in Zymomonas mobilis by elevating dehydrogenase activity and cofactor regeneration

Inhibitors

Recombinant strains

Cell growth (OD600)

Glucose consumption (g/L)

Ethanol production (g/L)

Inhibitor conversion (g/L)

Furfural

ZM4(pHW20a-ZMO1771)

0.259 ± 0.002

3.84 ± 0.11

1.14 ± 0.03

1.31 ± 0.25

 

ZM4(pHW20a-ZMO1771-pntAB)

0.252 ± 0.011

3.44 ± 0.09

1.04 ± 0.02

1.49 ± 0.03

 

ZM4(pHW20a-ZMO1771-udhA)

0.243 ± 0.006

4.02 ± 0.59

0.91 ± 0.05

1.57 ± 0.27

 

ZM4(pHW20a-ZMO1771-ZMO0367)

0.230 ± 0.005

3.52 ± 0.34

1.14 ± 0.07

1.42 ± 0.11

HMF

ZM4(pHW20a-ZMO1771)

0.357 ± 0.004

6.43 ± 0.76

1.99 ± 0.08

1.97 ± 0.18

 

ZM4(pHW20a-ZMO1771-pntAB)

0.421 ± 0.025

6.50 ± 0.13

2.28 ± 0.15

2.23 ± 0.14

 

ZM4(pHW20a-ZMO1771-udhA)

0.470 ± 0.036

7.84 ± 0.63

2.70 ± 0.08

2.72 ± 0.05

 

ZM4(pHW20a-ZMO1771-ZMO0367)

0.391 ± 0.009

5.66 ± 0.25

2.18 ± 0.16

2.02 ± 0.01

  1. In RM medium containing 2 g/L of furfural or 4 g/L of HMF at 30 °C. The fermentation performance was detected at the mid-log phase, where 12 h for furfural and 16 h for HMF. Standard deviations were derived from at least two independent determinations