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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: A novel small RNA CoaR regulates coenzyme A biosynthesis and tolerance of Synechocystis sp. PCC6803 to 1-butanol possibly via promoter-directed transcriptional silencing

Fig. 4

a Scheme of the GFP assays. b PCR validation of WT-GFP, CoaR(+)-GFP and CoaR(−)-GFP. Lane1: DNA ladder; Lane 2, 3, and 4: genomic DNA of WT-GFP, CoaR(+)-GFP and CoaR(−)-GFP as templates, respectively; primers Pslr0847-F and GFP-R were applied (Additional file 2: Table S3) and the target size was 1165 bp; Lane 5, 6, and 7: genomic DNA of WT-GFP, CoaR(+)-GFP, CoaR(−)-GFP, and WT as templates, respectively; primers 0168-F and 0168-R were applied (Additional file 2: Table S3) and the target size was approximately 3600 bp for WT-GFP, CoaR(+)-GFP and CoaR(−)-GFP, 500 bp for WT; c GFP fluorescence in the WT-GFP, CoaR(+)-GFP, and CoaR(−)-GFP strains. The error bars represent the calculated standard deviation of the measurements of three biological replicates

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