Fig. 1

Metabolic pathways for trans-nerolidol (sesquiterpene) production in yeast. ACS2 acetyl-CoA synthase, EfmvaE Enterococcus faecalis acetoacetyl-CoA thiolase/HMG-CoA reductase, EfmvaS E. faecalis HMG-CoA synthase, HMG2 HMG-CoA reductase 2, ERG12 mevalonate kinase, ERG8 phosphomevalonate kinase, MVD1 mevalonate pyrophosphate decarboxylase, IDI1 isopentenyl diphosphate:dimethylallyl diphosphate isomerase, ERG20 farnesyl pyrophosphate synthetase, AcNES1 Actinidia chinensis tran-nerolidol synthase, ERG9 squalene synthase, IPP isopentenyl pyrophosphate, DMAPP dimethylallyl pyrophosphate, FPP farnesyl pyrophosphate. Dashed arrow means multiple biochemical steps