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Table 1 S. cerevisiae strains used in this work

From: Coupling gene regulatory patterns to bioprocess conditions to optimize synthetic metabolic modules for improved sesquiterpene production in yeast

Strain

Genotype

Resource/reference

CEN.PK2-1C

MATa ura3-52 trp1-289 leu2-3,112 his3Δ 1

[44]

CEN.PK113-5D

MATa ura3-52

[44]

CEN.PK113-7D

MATa

[44]

ILHA series strains

 

oH5

oURA3 derivative; ERG9(1333, 1335)::yEGFP-CLN2 PEST-T URA3 -loxP-KlURA3-loxP

[23]

N6D

oH5 derivative; [pPMVAu8] [pPMVAd3] [pJT1]

[23]

NC1D

oH5 derivative; [pPMVAugw] [TRP1]a [pJT3]

This work

o391

CEN.PK2-1C derivative; HMG2 K6R(−152 ,−1)::HIS3-T EFM1 <EfmvaS<P GAL1 –P GAL10 >ACS2>T ACS2 –P GAL2 >EfmvaE>T EBS1 –P GAL7 ; pdc5 (−31, 94)::P GAL2 >ERG12>T NAT5 –P TEF2 >ERG8>T IDP1 –T PRM9 <MVD1<P ADH2 −T RPL15A <IDI1<P TEF1 -TRP1

This work

N391DA

o391 derivative; ERG9(1333, 1335)::CLN2 PEST-T URA3 -loxP-KlURA3-loxP gal80::loxP-kanMX4-loxP [pJT9R]

This work

oJ3

CEN.PK113-5D derivative; gal80::loxP-kanMX4-loxP

This work

GH4J3

oJ3 derivative; ura3 (1, 704)::KlURA3

This work

G89J3

oJ3 derivative; ura3 (1, 704)::KlURA3-P TEF1 -yEGFP

This work

GB5J3

oJ3 derivative; ura3 (1, 704)::KlURA3-P GAL1 -yEGFP

This work

GB6J3

oJ3 derivative; ura3 (1, 704)::KlURA3-P GAL10 -yEGFP

This work

GQ3J3

oJ3 derivative; ura3 (1, 704)::KlURA3-P GAL2 -yEGFP

This work

GQ4J3

oJ3 derivative; ura3 (1, 704)::KlURA3-P GAL7 -yEGFP

This work

  1. Symbol > or < indicates the direction of open reading frames
  2. a the plasmid pPMVAd36 was transformed