Table 2 Plasmids used in this work
Plasmid | Features | Reference |
|---|---|---|
pRS423 | E. coli/S. cerevisiae shuttle plasmid; 2 μ, HIS3 | [45] |
pRS424 | E. coli/S. cerevisiae shuttle plasmid; 2 μ, TRP1 | [45] |
pRS425 | E. coli/S. cerevisiae shuttle plasmid; 2 μ, LEU2 | [45] |
pPMVAu8 | pRS423: P RPL4A >EfmvaS>T EFM1 –P RPL15A >EfmvaE>T EBS1 | [23] |
pPMVAd3 | pRS424: P RPL8B >ERG12>T NAT5 –P SSB1 >ERG8>T IDP1 –P RPL3 >MVD1>T PRM9 –P YEF3 >IDI1>T RPL15A | [23] |
pJT1 | pRS425: P TEF2 >ERG20>T RPL3 –P TEF1 –AcNES1-T RPL41B | [23] |
pPMVAugw | pRS423: T EFM1 <EfmvaS<P CUP1 –P CUP1 >EfmvaE>T EBS1 | This work |
pIMVAu1 | pRS423: HMG2 (−309, −153)-HIS3-T EFM1 <EfmvaS<P GAL1 –P GAL10 >ACS2>T ACS2 –P GAL2 >EfmvaE>T EBS1 –P GAL7 >HMG2 K6R(1, 292) | This work |
pPMVAd36 | pRS424: P CUP1 >ERG12>T NAT5 –P TEF2 >ERG8>T IDP1 –T PRM9 <MVD1<P TEF2 –T RPL15A <IDI1<P TEF1 | This work |
pIMVAd39T | pUC19: pdc5 (−277, −32)-P GAL2 >ERG12>T NAT5 –P TEF2 >ERG8>T IDP1 –T PRM9 <MVD1<P ADH2 –T RPL15A <IDI1<P TEF1 -TRP1-pdc5 (95,373) | This work |
pJT3 | pRS425: T RPL3 <ScERG20<P CUP1 –P CUP1 >AcNES1>T RPL41B | This work |
pJT9R | pRS425: T RPL3 <ScERG20<P GAL1 –P GAL2 >AcNES1>T RPL41B | This work |
pILGFP3 | Yeast integration plasmid; yEGFP without promoter | [33] |
pILGH4 | pILGFP3: with yEGFP removed | [33] |
pILGB5A | pILGFP3: P GAL1 -yEGFP | [33] |
pILGB6 | pILGFP3: P GAL10 -yEGFP | This work |
pILGFPQ3 | pILGFP3: P GAL2 -yEGFP | This work |
pITGFPQ4 | pILGFP3: P GAL7 -yEGFP | This work |