Molecular and biochemical characterization of recombinant cel12B, cel8C, and peh28 overexpressed in Escherichia coli and their potential in biofuel production

Table 1 Isoelectric point (pI), molecular weight, and N-glycosylation sites predicted for cel12B, cel8C, and peh28

Enzyme	pI value	Molecular weight (kDa)^a	N-Glycosylation sites
Cel12B	9.17	29.5	Asn²²⁶, Asn²³⁰, Asn²³⁵, Asn²⁸⁰, Asn²⁸⁷, Asn³⁰⁸, Asn³³³. Asn³⁹³, Asn⁴⁴⁰
Peh28	9.46	41.5	Asn¹²⁸, Asn¹⁶¹, Asn¹⁶⁷, Asn²⁰⁷, Asn²⁵⁶
Cel8C	7.73	40	Asn¹²⁸

^aThe predicted molecular weights are in agreement with those identified for the enzymes using SDS-PAGE [8]

ISSN: 2731-3654