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Table 3 Exoglucanase, endoglucanase, and β-glucosidase activities of cel12B and cel8C on Avicel, CMC, and p-NPG, respectively

From: Molecular and biochemical characterization of recombinant cel12B, cel8C, and peh28 overexpressed in Escherichia coli and their potential in biofuel production

Enzyme Exoglucanase activity (U/ml)a Endoglucanase activity (U/ml) β-Glucosidase activity (U/ml)
Cel12B 1.53 ± 0.09 1.21 ± 0.02
Cel8C 14.7 ± 0.6
  1. The reactions were conducted for 1 h at 45 °C, pH 5.0 using 20 mg/ml Avicel or CMC for exoglucanase and endoglucanase activities, respectively, and for 30 min at 50 °C, pH 5.0 using 10 mM p-NPG for β-glucosidase activity
  2. All values are given as a mean of triplicates ± SE
  3. aA unit of enzyme activity (U) is defined as the amount of enzyme releasing 1 µmol of reducing sugars per minute for exoglucanase and endoglucanase activities on Avicel and CMC, respectively, and 1 µmol p-nitrophenol per minute for β-glucosidase activity on p-NPG under the assay conditions