Molecular and biochemical characterization of recombinant cel12B, cel8C, and peh28 overexpressed in Escherichia coli and their potential in biofuel production

Table 3 Exoglucanase, endoglucanase, and β-glucosidase activities of cel12B and cel8C on Avicel, CMC, and p-NPG, respectively

Enzyme	Exoglucanase activity (U/ml)^a	Endoglucanase activity (U/ml)	β-Glucosidase activity (U/ml)
Cel12B	1.53 ± 0.09	1.21 ± 0.02	–
Cel8C	–	14.7 ± 0.6	–

The reactions were conducted for 1 h at 45 °C, pH 5.0 using 20 mg/ml Avicel or CMC for exoglucanase and endoglucanase activities, respectively, and for 30 min at 50 °C, pH 5.0 using 10 mM p-NPG for β-glucosidase activity
All values are given as a mean of triplicates ± SE
^aA unit of enzyme activity (U) is defined as the amount of enzyme releasing 1 µmol of reducing sugars per minute for exoglucanase and endoglucanase activities on Avicel and CMC, respectively, and 1 µmol p-nitrophenol per minute for β-glucosidase activity on p-NPG under the assay conditions

ISSN: 2731-3654