Natural acetylation impacts carbohydrate recovery during deconstruction of Populus trichocarpa wood

Table 3 Dissolution and degradation of P. trichocarpa wood at various dilute acid pretreatment regimes

Regime	Time (min)	H₂SO₄	Dissolved sugars	Wood residue	Degraded	Xyl O:M	Glc O:M	Acetate
Regime	Time (min)	H₂SO₄	Dissolved sugars	Wood residue	Degraded	Xyl O:M	Glc O:M	AA	XOS	WR	AA:XOS:WR
1	10	0.0	33	930	ND	0.6	1.1	0.6	ND	50	1.0:ND:83
2	10	1.0	34	910	ND	1.8	1.1	1.4	ND	48	1.0:ND:34
3	10	3.0	33	880	ND	7.1	1.0	1.4	ND	50	1.0:ND:36
4	10	6.0	33	880	ND	3.2	1.2	1.6	ND	50	1.0:ND:31
5	30	0.0	38	880	ND	100	2.2	0.6	ND	50	1.0:ND:83
6	30	1.0	140	840	ND	4.5	2.1	3.0	12	41	1.0:4.0:13.7
7	30	3.0	280	740	ND	1.1	1.1	18	15	24	1.2:1.0:1.6
8	30	6.0	380	720	ND	0.5	0.5	37	ND	5.4	6.9:ND:1.0
9	60	0.0	280	720	ND	20	4.8	4.1	21	50	1.0:5.1:12.2
10	60	1.0	370	700	ND	0.4	0.3	40	5.5	3.0	13.3:1.8:1.0
11	60	3.0	340	690	74	0.2	0.2	54	ND	ND	1.0:ND:ND
12	60	6.0	210	680	230	0.8	0.3	58	ND	ND	1.0:ND:ND

Pretreatment temperature was 180 °C. H ₂ SO ₄ sulphuric acid, Glu glucose, Xyl xylose, O:M oligomer-to-monomer ratio; Acetate partitions into acetic acid (AA), dissolved acetate on xylooligosaccharides (XOS), and acetate on wood residue (WR). ND not detected (less than 5% w/w). Values are the average of three technical replicates. Soluble lignin and minor sugars arabinose, rhamnose and galactose are not included in the mass balance. All values except ratios are mg/g

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