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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Engineering vesicle trafficking improves the extracellular activity and surface display efficiency of cellulases in Saccharomyces cerevisiae

Fig. 4

Engineering the ER to Golgi vesicle trafficking improved the display efficiency of heterologous proteins. a The cell activity of CelA in the ER to Golgi vesicle trafficking engineered strains. b The cell activity of BGL1 in the ER to the Golgi vesicle trafficking engineered strains. c FACS analysis of CelA display efficiency in the ER to Golgi vesicle trafficking engineered strains. Control: the strains without expressing heterologous protein was used as the negative control. The black bars represent 36 h, the gray bars represent 72 h. The data are presented as the means ± standard errors from two independent experiments. *p value of the marked sample vs. control (PYX) <0.05

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