|
Metabolite
|
Response ratio (Producer/WT)
|
T test significance
|
|---|
|
Day 3
|
Day 7
|
Day 3
|
Day 7
|
|---|
|
Log2
|
Log2
|
P < 0.05
|
P < 0.05
|
|---|
|
Alanine
|
−0.864
|
−1.083
|
1.0E−02
|
8.3E−05
|
|
Isoleucine
|
−0.725
|
−1.171
|
1.4E−03
|
3.0E−05
|
|
Valine
|
−0.677
|
−1.736
|
4.7E−03
|
5.8E−05
|
|
Glutamic acid
|
−0.506
|
−0.414
|
1.1E−02
|
8.2E−05
|
|
Glutamic acid, N-acetyl-
|
−0.681
|
−0.223
|
2.8E−06
|
1.9E−03
|
|
Glutamine (pyroglutamate, glutamate)a
|
−0.480
|
−0.544
|
6.7E−04
|
1.2E−04
|
|
Succinate
|
−0.435
|
−0.624
|
3.0E−03
|
2.1E−06
|
|
Succinate semialdehyde
|
−0.763
|
−1.075
|
2.8E−07
|
9.8E−09
|
|
Glyceric acid
|
−1.739
|
−1.410
|
5.2E−05
|
5.7E−05
|
|
Glycerophosphoglycerol
|
−0.860
|
−0.900
|
1.2E−05
|
1.7E−07
|
|
Glycine
|
1.741
|
2.954
|
8.6E−07
|
1.1E−05
|
|
A231002
|
−0.474
|
−0.868
|
2.0E−03
|
7.8E−07
|
|
A300001
|
0.603
|
0.468
|
3.2E−03
|
1.8E−02
|
- Log2-transformed initial changes, i.e. response ratios of producer over wild type (WT), at low ethanol concentration (Fig. 1), were calculated and significance tested P < 0.05 using the t test (italics font: P < 0.0001)
-
aPyroglutamic acid represents the sum of the glutamine, pyroglutamic and glutamic acid pool
