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Table 1 Phase I changes of primary metabolism comparing the Synechococcus 7002 producer to WT

From: Systems analysis of ethanol production in the genetically engineered cyanobacterium Synechococcus sp. PCC 7002

Metabolite Response ratio (Producer/WT) T test significance
Day 3 Day 7 Day 3 Day 7
Log2 Log2 P < 0.05 P < 0.05
Alanine −0.864 −1.083 1.0E−02 8.3E−05
Isoleucine −0.725 −1.171 1.4E−03 3.0E−05
Valine −0.677 −1.736 4.7E−03 5.8E05
Glutamic acid −0.506 −0.414 1.1E−02 8.2E05
Glutamic acid, N-acetyl- −0.681 −0.223 2.8E06 1.9E−03
Glutamine (pyroglutamate, glutamate)a −0.480 −0.544 6.7E−04 1.2E−04
Succinate −0.435 −0.624 3.0E−03 2.1E06
Succinate semialdehyde −0.763 −1.075 2.8E07 9.8E09
Glyceric acid −1.739 −1.410 5.2E05 5.7E05
Glycerophosphoglycerol −0.860 −0.900 1.2E05 1.7E07
Glycine 1.741 2.954 8.6E07 1.1E05
A231002 −0.474 −0.868 2.0E−03 7.8E07
A300001 0.603 0.468 3.2E−03 1.8E−02
  1. Log2-transformed initial changes, i.e. response ratios of producer over wild type (WT), at low ethanol concentration (Fig. 1), were calculated and significance tested P < 0.05 using the t test (italics font: P < 0.0001)
  2. aPyroglutamic acid represents the sum of the glutamine, pyroglutamic and glutamic acid pool